Human Hemoglobin Antibody
R&D Systems | Catalog # G-134-C
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Human Hemoglobin
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Hemoglobin Antibody
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Hemoglobin localization in control and ALS spinal cord. Immunohistochemical staining of hemoglobin extravasation in cervical white matter from control (a–c) or ALS (d–f) spinal cord. Hemoglobin immunoreactivity (red) and lectin-positive vessels (green) are shown with a Hoechst nuclear counterstain (blue). Scale bars = 50 µm. Representative images of full spinal cord sections from the cervical level of a control case with no hemoglobin leakage (g), the thoracic level of an ALS case with white matter hemoglobin leakage (h), and the lumbar level of an ALS case with gray matter hemoglobin leakage (i) are shown. Dashed lines show gray matter boundary; scale bar = 1 mm. Occasional hemoglobin staining of SMI-32-positive anterior horn motor neurons was also observed (j–m). White arrowhead indicates a SMI-32-positive hemoglobin-positive motor neuron, where the red arrow indicates an SMI-32-positive hemoglobin-negative motor neuron. Scale bars = 50 µm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Hemoglobin localization in control and ALS spinal cord. Immunohistochemical staining of hemoglobin extravasation in cervical white matter from control (a–c) or ALS (d–f) spinal cord. Hemoglobin immunoreactivity (red) and lectin-positive vessels (green) are shown with a Hoechst nuclear counterstain (blue). Scale bars = 50 µm. Representative images of full spinal cord sections from the cervical level of a control case with no hemoglobin leakage (g), the thoracic level of an ALS case with white matter hemoglobin leakage (h), and the lumbar level of an ALS case with gray matter hemoglobin leakage (i) are shown. Dashed lines show gray matter boundary; scale bar = 1 mm. Occasional hemoglobin staining of SMI-32-positive anterior horn motor neurons was also observed (j–m). White arrowhead indicates a SMI-32-positive hemoglobin-positive motor neuron, where the red arrow indicates an SMI-32-positive hemoglobin-negative motor neuron. Scale bars = 50 µm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Hemoglobin localization in control and ALS spinal cord. Immunohistochemical staining of hemoglobin extravasation in cervical white matter from control (a–c) or ALS (d–f) spinal cord. Hemoglobin immunoreactivity (red) and lectin-positive vessels (green) are shown with a Hoechst nuclear counterstain (blue). Scale bars = 50 µm. Representative images of full spinal cord sections from the cervical level of a control case with no hemoglobin leakage (g), the thoracic level of an ALS case with white matter hemoglobin leakage (h), and the lumbar level of an ALS case with gray matter hemoglobin leakage (i) are shown. Dashed lines show gray matter boundary; scale bar = 1 mm. Occasional hemoglobin staining of SMI-32-positive anterior horn motor neurons was also observed (j–m). White arrowhead indicates a SMI-32-positive hemoglobin-positive motor neuron, where the red arrow indicates an SMI-32-positive hemoglobin-negative motor neuron. Scale bars = 50 µm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Neurovascular unit component expression in leaked versus non-leaked vessels along the spinal cord axis. Immunohistochemical labelling of neurovascular unit markers in ALS spinal cord (a–l); tight junctions claudin-5 (a, g) and ZO-1 (b, h), efflux pump P-glycoprotein (c, i), basement membrane marker collagen IV (d, j), and astrocyte markers aquaporin 4 (e, k) and GFAP (f, l), in spinal cord vessels with or without hemoglobin leakage. Scale bar = 50 µm. Automated quantification of average intensity staining of neurovascular unit markers was carried out in all ALS cases (n = 13) or in a subset of ALS cases with high hemoglobin leakage (n = 5) in leaked and non-leaked areas of the white and gray matter of the spinal cord (m–r). Composite of original images showing anti-hemoglobin immunoreactivity (red) and lectin-positive vessels (green) (m) and overlays of hemoglobin leakage analysis output (white, partly transparent) over anti-hemoglobin (red) (n). Segmentation of vessels inside (magenta) or outside (white) areas of hemoglobin leakage in the white matter (o) or gray matter (p). Dashed lines show boundaries. Scale bar = 1 mm. Perivascular astrocyte endfeet staining (green) around lectin-positive vessels (white) in (q) was isolated using an automated mask of the glia limitans (r). Scale bar = 50 µm. The average intensities of marker staining were measured in leaked and non-leaked vessels of the gray and white matter (s–x). Data shown as mean ± SD (n = 5 or 13) with statistical significance determined with a two-way repeated-measures ANOVA with Sidak’s post-test. ns = not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Hemoglobin by Immunohistochemistry
Hemoglobin localization in control and ALS spinal cord. Immunohistochemical staining of hemoglobin extravasation in cervical white matter from control (a–c) or ALS (d–f) spinal cord. Hemoglobin immunoreactivity (red) and lectin-positive vessels (green) are shown with a Hoechst nuclear counterstain (blue). Scale bars = 50 µm. Representative images of full spinal cord sections from the cervical level of a control case with no hemoglobin leakage (g), the thoracic level of an ALS case with white matter hemoglobin leakage (h), and the lumbar level of an ALS case with gray matter hemoglobin leakage (i) are shown. Dashed lines show gray matter boundary; scale bar = 1 mm. Occasional hemoglobin staining of SMI-32-positive anterior horn motor neurons was also observed (j–m). White arrowhead indicates a SMI-32-positive hemoglobin-positive motor neuron, where the red arrow indicates an SMI-32-positive hemoglobin-negative motor neuron. Scale bars = 50 µm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34446086), licensed under a CC-BY license. Not internally tested by R&D Systems.Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL.
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Formulation
Lyophilized from a 0.2 μm filtered solution in NaH<SUB>2</SUB>PO<SUB>4</SUB>, NaCl and Sodium Azide with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Hemoglobin
Alternate Names
alpha one globin;alpha-1 globin;alpha-1-globin;alpha-2 globin chain;Alpha-globin;ALPHA-GLOBIN GENE;delta globin;Haemoglobin;Hb;HbA1c;hemoglobin alpha 1 globin chain;Hemoglobin alpha chain;Hemoglobin subunit alpha;hemoglobin, alpha 1
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Product Documents for Human Hemoglobin Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Hemoglobin Antibody
For research use only
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Citations for Human Hemoglobin Antibody
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