Human HNF-4 alpha/NR2A1 Isoform 7-9 Antibody (H6939)

Detection of fbr9nfbuet5uiwgohdtn.jpg by Flow Cytometry

The effects of both P1- and P2-HNF4A on malignant phenotypes of gastric cancer cells in vitro. (A) P1-HNF4A or P2-HNF4A overexpression lentivirus were transfected into SGC7901 and BGC823 cells. Cell proliferation was examined using the CCK8 assay. OD450 absorbance was detected every day for a continuous 4 days (**P < 0.01, n.s., nonsignificant, n = 3). (B) The effects of P1- and P2-HNF4A overexpression on SGC7901 and BGC823 cell cycles using a flow cytometry assay (**P < 0.01; n.s., nonsignificant, n = 3). (C) The effects of both P1- and P2-HNF4A overexpression on SGC7901 and BGC823 cell migrations using transwell experiments. Migrated cells were counted using ImageJ (**P < 0.01; n.s., nonsignificant, n = 3) (Crystal Violet staining, 20×). (D) The effects of both P1- and P2-HNF4A overexpression on SGC7901 and BGC823 cell invasion using transwell experiments. Invaded cells were counted by ImageJ (**P < 0.01; n.s., nonsignificant, n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33710810), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of HNF-4 alpha/NR2A1 by Western Blot

HNF4 alpha isoforms differentially impact diurnal gene expression. (A) Heatmap of rlog read counts for all circadian-regulated and core clock genes with padj ≤ 0.01 and log2FC ≥ 2 between any pair of time points. 10:30, ZT3.5; 13:30, ZT6.5; 20:30 ZT13.5 (B) Average FPKM of select circadian clock genes. # padj ≤ 0.05; * padj ≤ 0.01. (C) Sample distance matrix for each RNA-seq replicate, calculated across the full transcriptome. The darker the color, the higher the degree of similarity. (D) Relative fold increase in P1- and P2-HNF4 alpha in livers of WT or littermate Clock KO by qRT-PCR at indicated ZT. Two-way ANOVA, Sidak’s multiple comparisons test, # p ≤ 0.05; ** p ≤ 0.005. Error bars, SEM (n=3-4). (E) Representative IB of diurnal expression of P2-HNF4 alpha protein in WCE from WT and Clock KO livers using P2-specific antibodies (n=3-4). See also Supplementary Figure 5. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fendo.2023.1266527/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of HNF-4 alpha/NR2A1 by Immunohistochemistry

The roles of both P1- and P2-HNF4A on gastric cancer (GC) progression in vivo using murine xenograft experiments. (A) BALB/c nude mice underwent orthotopic implantation with SGC7901 cells stably transfected by a negative control, P1-HNF4A, and P2-HNF4A overexpression lentiviruses. (B) The tumors were extracted and measured. Tumor volume was calculated using the formula (V = length × width2 × 0.5). The results are expressed as the mean ± SEM (*P < 0.05, n.s., nonsignificant). (C) H&E staining of sections from orthotopic specimens. Scar bars = 200 μm and 50 μm). (D) Ki67 staining of section from orthotopic specimens by immunohistochemistry. Scale bars = 200 μm and 50 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33710810), licensed under a CC-BY license. Not internally tested by R&D Systems.