Human HNF-4 alpha/NR2A1 Antibody (K9218)

R&D Systems | Catalog # PP-K9218-00

Isoforms 1-6
R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # K9218
View all HNF-4 alpha/NR2A1 Primary Antibodies »

Product Specifications

Immunogen

Human HNF-4 alpha/NR2A1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human HNF-4 alpha/NR2A1 Antibody (K9218)

Detection of HNF-4 alpha/NR2A1 by Flow Cytometry

Detection of HNF-4 alpha/NR2A1 by Flow Cytometry

DKK2 is required for Lgr5 positive stem cells-driven liver metastasis of colorectal cancer.(A) Isolated cells from AKP tumor organoids expressing the tdTomato reporter gene were dissociated and injected into the spleen of wild-type C57BL/6 mice. 8 weeks after the injection, metastatic tumor growth was measured by in vivo imaging analysis. (B) Representative pictures of in vivo imaging analysis. Ctrl: AKP control organoids transduced with scrambled guide RNA, KO: Dkk2 knockout AKP organoids. (C–D) Statistic analysis of liver metastasis (C) and survival (D). Ctrl (n=10), KO (n=15), n represents the number of mice. c.p.m. in (C): count per minute. (E) Quantitative gene expression analysis of Dkk2, Lgr5, and Hnf4 alpha 1 in liver metastasized colon cancer cells. Ctrl (n=11), KO (n=12), n represents the number of isolated cancer nodules with five mice per group. (F) Representative flow cytometry analysis data of c-Src phosphorylation (pSrc) and Lgr5 expression in metastasized colon cancer cells. (G) Statistic analysis of Lgr5 expression and pSrc in (E). The average of mean fluorescence intensity in control samples was set as 1 and relative fluorescence intensity was calculated. (H) Statistic analysis of the percentile of Lgr5 high (Lgr5 and pSrc double positive) cells in metastasized colon cancer in (F). Each symbol represents an individually isolated cancer nodule. n.s.=not significant, **p<0.01, ****p<0.0001; two-tailed Welch’s t-test (C, E, G, H). Error bars indicate mean ± s.d. Log-rank test (D). Results are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39535280), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human HNF-4 alpha/NR2A1 Antibody (K9218)

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using PP-K9218-00 in the following applications:

Formulation, Preparation, and Storage

Formulation

A liquid formulation in physiologic saline with 0.1% NaN<sub>3</sub>.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: HNF-4 alpha/NR2A1

Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

Additional HNF-4 alpha/NR2A1 Products

Product Documents for Human HNF-4 alpha/NR2A1 Antibody (K9218)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HNF-4 alpha/NR2A1 Antibody (K9218)

For research use only

Citations for Human HNF-4 alpha/NR2A1 Antibody (K9218)

Customer Reviews for Human HNF-4 alpha/NR2A1 Antibody (K9218) (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human HNF-4 alpha/NR2A1 Antibody (K9218)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human HNF-4 alpha/NR2A1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Pancreas tissue
    Species: Human
    Verified Customer | Posted 11/22/2022
    Human HNF-4 alpha/NR2A1 Antibody (K9218) PP-K9218-00
  • Human HNF-4 alpha/NR2A1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Hepatocytes
    Species: Human
    Verified Customer | Posted 12/17/2021
    Human HNF-4 alpha/NR2A1 Antibody (K9218) PP-K9218-00

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail