Human Iduronate 2-Sulfatase/IDS Antibody

R&D Systems | Catalog # AF2449

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Iduronate 2-Sulfatase/IDS Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IDS
Ser26-Pro550
Accession # P22304

Specificity

Detects human IDS in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IDS is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Iduronate 2-Sulfatase/IDS Antibody

Detection of Human Iduronate 2-Sulfatase/IDS antibody by Western Blot.

Detection of Human Iduronate 2‑Sulfatase/IDS by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Iduronate 2-Sulfatase/IDS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2449) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Iduronate 2-Sulfatase/IDS at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Iduronate 2-Sulfatase/IDS Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Iduronate 2-Sulfatase/IDS (Catalog # 2449-SU), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 1 review rated 4 using AF2449 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Iduronate 2-Sulfatase/IDS

As a member of the sulfatase family, IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate (2, 3). It hydrolyzes the 2‑sulfate group of the L-iduronate 2-sulfate units of the GAG. The IDS deficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome), an X-linked inborn error leading to lysosomal accumulation of the GAG and its excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe. The deduced amino acid sequence of human IDS consists of a signal peptide (residues 1-25), a pro peptide (residues 26-33) and a mature chain (residues 34-550) that may be further processed into the 42 kDa chain (residues 34-455) and the 14 kDa chain (residues 456-550) (1). Recombinant human IDS corresponds to the single chain and has sulfatase activity described above.

References

  1. Wilson, P.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8531.
  2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
  3. Neufeld, E.F. and J. Muenzer (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) pp. 3421-3452, New York, McGraw-Hill.

Alternate Names

SIDS

Entrez Gene IDs

3423 (Human); 15931 (Mouse)

Gene Symbol

IDS

UniProt

P22304

Additional Iduronate 2-Sulfatase/IDS Products

Product Documents for Human Iduronate 2-Sulfatase/IDS Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Iduronate 2-Sulfatase/IDS Antibody

For research use only

Citations for Human Iduronate 2-Sulfatase/IDS Antibody

Customer Reviews for Human Iduronate 2-Sulfatase/IDS Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 19602578
    Species: Rat
    Verified Customer | Posted 01/07/2015

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