Human Iduronate 2-Sulfatase/IDS Antibody

Detection of Human Iduronate 2‑Sulfatase/IDS by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Iduronate 2-Sulfatase/IDS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2449) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Iduronate 2-Sulfatase/IDS at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Iduronate 2-Sulfatase by Immunocytochemistry/ Immunofluorescence

Levels of iduronate-2 sulfatase (IDS) are decreased in cancer epithelia in vivo and in culture. (A) Scatter plot of dermatan sulfate (DS) levels normalized to total protein measured using ELISA in HMLE, MCF-7 and MDA-MB-231 cells cultured in 3D Type 1 collagen scaffolds. (B) Scatter plot of IDS mRNA levels in HMLE, MCF-7 and MDA-MB-231 cells cultured in 3D Type 1 collagen scaffolds, as determined by real time PCR with 18S rRNA as internal control. (C) (left) Epifluorescence micrographs of matched normal breast sections (top) and breast cancer tissues (bottom) stained for DNA (using DAPI; blue), and IDS (using antibody; red), scale = 200 μm. (middle) 3D profile plots of IDS levels in normal (top) and cancer (bottom) sections with bright colored peaks showing higher staining for IDS in nontransformed cells. (right) Bar graph showing a statistically significant decrease in IDS levels in breast cancer tissues compared with adjacent breast epithelial cells (50 cells, 5 fields, 2 sample sets). (D) Confocal micrographs of HMLE, MCF-7 and MDA-MB-231 cells cultured on top of Type 1 collagen scaffolds and stained for DNA (with DAPI; blue), F-actin (with phalloidin; pink), acidic compartment (with antibody against LAMP2; green), and for IDS (antibody; red), scale = 20 µm. Significance was measured using one-way ANOVA and student’s t test (* p < 0.05, ** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31574977), licensed under a CC-BY license. Not internally tested by R&D Systems.