Human IGF-I/IGF-1 Antibody (56408)

R&D Systems | Catalog # MAB291

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Microarray Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 56408
View all IGF-I/IGF-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IGF-I/IGF-1
Gly49-Ala118
Accession # P01343

Specificity

Detects human IGF-I/IGF-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) IGF-I/IGF-1, rmIGF‑II/IGF-2, or recombinant human IGF‑II/IGF-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IGF-I/IGF-1 Antibody (56408)

Detection of Recombinant Human IGF-I/IGF-1 antibody by Western Blot.

Detection of Recombinant Human IGF-I/IGF-1 by Western Blot.

Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Human IGF-I ELISA Standard Curve

Human IGF-I ELISA Standard Curve

Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) was serially diluted and captured by Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IGF-I/IGF-1 Antibody (56408)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)

Human IGF-I/IGF-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IGF-I/IGF-1 Biotinylated Antibody (Catalog # BAF291)
  • Standard: Recombinant Human IGF-I/IGF-1 Protein, CF (Catalog # 291-G1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB291 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I/IGF-1

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

UniProt

P01343

Additional IGF-I/IGF-1 Products

Product Documents for Human IGF-I/IGF-1 Antibody (56408)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IGF-I/IGF-1 Antibody (56408)

For research use only

Citations for Human IGF-I/IGF-1 Antibody (56408)

Customer Reviews for Human IGF-I/IGF-1 Antibody (56408) (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human IGF-I/IGF-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: HE49 cells and cells medium
    Species: Human
    Verified Customer | Posted 06/18/2021
    Samples were normalized to cell number and the total volume of medium
    Human IGF-I/IGF-1 Antibody (56408) MAB291
  • Human IGF-I/IGF-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: decidual
    Species: Human
    Verified Customer | Posted 04/10/2021
    Low primary antibody dilution (1:500) for IHC.
    Human IGF-I/IGF-1 Antibody (56408) MAB291

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