Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity.
IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.
Human IL-18 BPa Antibody (136031)
R&D Systems | Catalog # MAB1192
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
Immunohistochemistry, ELISA Capture
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 136031
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-18 BPa
Thr31-Gly194 (predicted)
Accession # O95998
Thr31-Gly194 (predicted)
Accession # O95998
Specificity
Detects human IL-18 BPa in ELISAs. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) IL-18, recombinant mouse (rm) IL-18 BPc and rmIL-18 BPd is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-18 BPa Antibody (136031)
Human IL-18 BPa ELISA Standard Curve
Recombinant Human IL‑18 BPa Fc Chimera (Catalog # 119-BP) was serially diluted and captured by Mouse Anti-Human IL‑18 BPa Monoclonal Antibody (Catalog # MAB1192) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human IL-18 BPa Antibody (136031)
Human IL-18 BPa Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB1192 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-18 BPa
References
- Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
- Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
- Calderara, S. et al. (2001) Virology 279:22.
Long Name
Interleukin 18 Binding Protein Isoform a
Alternate Names
IL18 BPa, IL18BP
Gene Symbol
IL18BP
UniProt
Additional IL-18 BPa Products
Product Documents for Human IL-18 BPa Antibody (136031)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL-18 BPa Antibody (136031)
For research use only
Related Research Areas
Citations for Human IL-18 BPa Antibody (136031)
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Application: ELISASample Tested: Cell culture supernatantSpecies: HumanVerified Customer | Posted 11/09/2017MAB1192 was used as the capture and AF119 was used as the detection antibody for the sandwich ELISA for IL-18PB. The immunoassay standard was 119-BP. Assay had sensitivity of ~10pg/ml.
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