Human IL-1 alpha/IL-1F1 Antibody [Biotin]

R&D Systems | Catalog # BAF200

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry

Cited:

Electrochemiluminescent Assay, ELISA Development, ELISA Microarray Development, Functional Assay, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IL-1 alpha/IL-1F1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-1 alpha /IL-1F1 (R&D Systems, Catalog # 200-LA)
Ser113-Ala271
Accession # Q53QF9

Specificity

Detects human IL-1 alpha /IL-1F1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse IL‑1 alpha, recombinant porcine IL‑1 alpha, recombinant rat IL‑1 alpha, recombinant human (rh) IL-1 sRI, and rhIL-1 sRII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL-1 alpha/IL-1F1 Antibody [Biotin]

IL-1a/IL-1F1 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑1 alpha /IL‑1F1 in Human PBMCs.

IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Human IL-1a/IL-1F1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF200) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IL-1a/IL-1F1 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑1 alpha /IL‑1F1 in Human PBMCs.

IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Human IL-1a/IL-1F1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF200) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Human IL-1 alpha / IL-1F1 ELISA Standard Curve

Human IL-1 alpha / IL-1F1 ELISA Standard Curve

Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) was serially diluted and captured by Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB200) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IL-1 alpha/IL-1F1 Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with LPS and monensin

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA)

Human IL-1 alpha /IL-1F1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human IL‑1 alpha /IL‑1F1 Antibody (Catalog # MAB200)
  • Capture Reagent: Human IL‑1 alpha /IL‑1F1 Antibody (Catalog # MAB200R)
  • Standard: Recombinant Human IL-1 alpha/IL-1F1 Protein (Catalog # 200-LA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF200 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts, and glia (oligodendroglia, astrocytes, and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Entrez Gene IDs

3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)

Gene Symbol

IL1A

UniProt

Q53QF9

Additional IL-1 alpha/IL-1F1 Products

Product Documents for Human IL-1 alpha/IL-1F1 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-1 alpha/IL-1F1 Antibody [Biotin]

For research use only

Citations for Human IL-1 alpha/IL-1F1 Antibody [Biotin]

Customer Reviews for Human IL-1 alpha/IL-1F1 Antibody [Biotin] (1)

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  • Human IL-1 alpha /IL-1F1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 08/20/2021
    This antibody was used as the ELISA detection.
    Human IL-1 alpha/IL-1F1 Antibody [Biotin] BAF200

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Protocols

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Associated Pathways

IL-1 Family Signaling Pathways
IL-1 Family Signaling Pathway Thumbnail