Human IL-1 beta/IL-1F2 Antibody [Biotin]

Name: Human IL-1 beta/IL-1F2 Antibody [Biotin]
Brand: R&D Systems
SKU: BAF201
Price: 589 USD
Availability: InStock

R&D Systems | Catalog # BAF201

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Citations (56)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Antibody Array Development, Array Development, Bioassay, Electrochemiluminescent Assay, ELISA Detection, ELISA Development, Functional Assay, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-1 beta /IL-1F2
Ala117-Ser269
Accession # NP_000567

Specificity

Detects human IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 4% cross-reactivity with recombinant ratIL‑1 beta is observed and less than 0.1% cross-reactivity with recombinant mouse IL‑1 beta and recombinant porcine IL‑1 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL-1 beta/IL-1F2 Antibody [Biotin]

Detection of IL‑1 beta /IL‑1F2 in THP‑1 Human Cell Line.

IL‑1 beta /IL‑1F2 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Goat Anti-Human IL‑1 beta /IL‑1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 5 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of THP-1 cells treated with 200nM PMA for 24 hours then 10ug/mL LPS for 24 hours. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

IL‑1 beta /IL‑1F2 in Human PBMCs.

IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-1 beta /IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL‑1 beta /IL‑1F2 in Human PBMCs.

IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-1 beta /IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human IL-1 beta/IL-1F2 by Functional

Differences in Functional Profiles between Three Patients with Different Clinical Outcomesgp100 209–2M spots co-spotted with anti-IFN gamma, anti-TNF alpha, anti-IL1b, and anti-IL-6 are shown for three separate patient samples. Patient 10735, who remains free of disease at this writing, displays strong IFN gamma, TNF alpha, IL-1b, and IL-6 secretion. Patients 10710 and 10737 show strong IFN gamma secretion, but weak to no TNF alpha, IL-1b, and IL-6 secretion; both patients experienced disease recurrence soon after these samples were drawn. Note the diffuse pattern of IL-1b and IL-6 capture, which differs from the focal capture of IFN gamma and TNF alpha. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/16162034), licensed under a CC-BY license. Not internally tested by R&D Systems.

Human IL-1 beta / IL-1F2 ELISA Standard Curve

Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) was serially diluted and captured by Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

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Applications for Human IL-1 beta/IL-1F2 Antibody [Biotin]

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) and THP-1 human acute monocytic leukemia cell line untreated and treated with PMA and LPS

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB)

Human IL-1 beta /IL-1F2 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human IL‑1 beta /IL‑1F2 Antibody (Catalog # MAB601)
Capture Reagent: Human IL‑1 beta /IL‑1F2 Antibody (Catalog # MAB601R)
Standard: Recombinant Human IL-1 beta/IL-1F2 Protein (Catalog # 201-LB)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.