Human IL-34 Antibody (578401) [Biotin]

R&D Systems | Catalog # BAM52652

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 578401
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-34
Asn21-Pro242
Accession # NP_689669

Specificity

Detects human IL-34 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse IL-34 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human IL-34 Antibody (578401) [Biotin]

Human IL-34 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human IL‑34 Antibody (Catalog # MAB52651)
  • Standard: Recombinant Human IL-34 Protein (Catalog # 5265-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-34

Interleukin 34 (IL-34) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. The mature chain contains one potential site for N-linked glycosylation. IL-34 is widely expressed, but it is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). Functionally, IL-34 stimulates monocyte viability.

Long Name

Interleukin 34

Alternate Names

C16orf77, IL34

Entrez Gene IDs

146433 (Human); 76527 (Mouse)

Gene Symbol

IL34

UniProt

Additional IL-34 Products

Product Documents for Human IL-34 Antibody (578401) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-34 Antibody (578401) [Biotin]

For research use only

Related Research Areas

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