Human Integrin alpha L/CD11a Antibody (CR38)

Name: Human Integrin alpha L/CD11a Antibody (CR38)
Brand: R&D Systems
SKU: MAB35951
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB35951

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # CR38

View all Integrin alpha L/CD11a Primary Antibodies »

Product Specifications

Immunogen

Fibronectin purified human monocytes

Specificity

Detects human Integrin alpha L/CD11a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Integrin alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6, alpha 8, alpha 9, alpha 10, alpha 11, alpha M, alpha V, alpha X, rhIntegrin beta 3, rhIntegrin alpha 6(x1)+ beta 4, alpha 7(x2)+ beta 1, alpha D+ beta 2, alpha E+ beta 7, recombinant mouse Integrin alpha 2b or alpha L is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Integrin alpha L/CD11a Antibody (CR38)

Detection of Integrin alpha L/CD11a in Human peripheral blood monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Human Integrin aL/CD11a Monoclonal Antibody (Catalog # MAB35951, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Applications for Human Integrin alpha L/CD11a Antibody (CR38)

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood monocytes

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin alpha L/CD11a

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.