Human Integrin beta 4/CD104 Antibody (422325)

R&D Systems | Catalog # MAB4060

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, ELISA Capture, ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 422325
View all Integrin beta 4/CD104 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 4/CD104
Asn28-Ser710
Accession # P16144

Specificity

Detects human Integrin beta 4/CD104 in Western blots. In Western blots, no cross-reactivity with recombinant human Integrin  beta 1, beta 2, beta 3, beta 5, beta 6, beta 8, or recombinant mouse Integrin  beta 4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Integrin beta 4/CD104 Antibody (422325)

Integrin beta 4/CD104 antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

Integrin beta 4/CD104 in A431 Human Cell Line.

Integrin beta 4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4060) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Integrin beta 4/CD104 Antibody (422325)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Integrin  beta 4/CD104 under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 3.5 using MAB4060 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Integrin beta 4/CD104

Integrin beta 4 (also GP150 and CD104) is a 150‑200 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta 4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. Two ECD alternative splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta 4 ECD shares 88% aa sequence identity with mouse beta 4 ECD.

Alternate Names

CD104, ITGB4

Entrez Gene IDs

3691 (Human); 192897 (Mouse); 25724 (Rat)

Gene Symbol

ITGB4

UniProt

P16144

Additional Integrin beta 4/CD104 Products

Product Documents for Human Integrin beta 4/CD104 Antibody (422325)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Integrin beta 4/CD104 Antibody (422325)

For research use only

Citations for Human Integrin beta 4/CD104 Antibody (422325)

Customer Reviews for Human Integrin beta 4/CD104 Antibody (422325) (2)

3.5 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
50%
3 Stars
50%
2 Stars
0%
1 Stars
0%

Have you used Human Integrin beta 4/CD104 Antibody (422325)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human Integrin  beta 4/CD104 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/13/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Integrin beta 4/CD104 Antibody (422325) MAB4060
  • Human Integrin  beta 4/CD104 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies