Human Integrin beta 6 Antibody

R&D Systems | Catalog # AF4155

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Integrin beta 6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Integrin beta 6
Gly22-Asn707
Accession # P18564

Specificity

Detects human Integrin  beta 6 in the direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse Integrin  beta 6 is observed and less than 5% cross-reactivity with recombinant human (rh) Integrin  beta 3 and rhIntegrin  beta 5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Integrin beta 6 Antibody

Detection of Integrin beta 6 antibody in HT‑29 Human Cell Line antibody by Flow Cytometry.

Detection of Integrin beta 6 in HT‑29 Human Cell Line by Flow Cytometry.

HT-29 human colon adenocarcinoma cell line was stained with Sheep Anti-Human Integrin beta 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155, filled histogram) or isotype control antibody (5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
Integrin beta 6 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

Integrin beta 6 in HT‑29 Human Cell Line.

Integrin beta 6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Sheep Anti-Human Integrin beta 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Integrin  beta 6 in HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells.

Integrin  beta 6 was detected in immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells using Sheep Anti-Human Integrin  beta 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Integrin beta 6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells 

Western Blot

0.1 µg/mL
Sample: Recombinant Human Integrin  beta 6

Reviewed Applications

Read 1 review rated 5 using AF4155 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin beta 6

Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta 6 ECD, respectively.

Alternate Names

ITGB6

Entrez Gene IDs

3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)

Gene Symbol

ITGB6

UniProt

P18564

Additional Integrin beta 6 Products

Product Documents for Human Integrin beta 6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin beta 6 Antibody

For research use only

Citations for Human Integrin beta 6 Antibody

Customer Reviews for Human Integrin beta 6 Antibody (1)

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  • Human Integrin  beta 6 Antibody
    Name: Preethi Vijayaraj
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 03/12/2018
    Human Integrin beta 6 Antibody AF4155

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Protocols

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