Human Jagged 2 Antibody

R&D Systems | Catalog # AF1726

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Jagged 2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Jagged 2
Met27-Asp307
Accession # Q9Y219

Specificity

Detects human Jagged 2 in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant human Jagged 1 is observed and less than 1% cross-reactivity with recombinant rat Jagged 1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Jagged 2 Antibody

Jagged 2 antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

Jagged 2 in HUVEC Human Cells.

Jagged 2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Jagged 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Jagged 2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle

Western Blot

0.1 µg/mL
Sample: Recombinant Human Jagged 2 Fc Chimera (Catalog # 1726-JG)

Reviewed Applications

Read 3 reviews rated 4 using AF1726 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jagged 2

Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1-5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421-461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2 week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).

References

  1. Shawber, C. et al. (1996) Dev. Biol. 180:370.
  2. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
  3. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
  4. Schickwann, T. et al. (2000) Blood 96:950.
  5. DeHart, S. et al. (2005) Blood 105:3521.
  6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.

Alternate Names

HJ2, JAG2

Entrez Gene IDs

3714 (Human); 16450 (Mouse); 29147 (Rat)

Gene Symbol

JAG2

UniProt

Q9Y219 (Human)

Additional Jagged 2 Products

Product Documents for Human Jagged 2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Jagged 2 Antibody

For research use only

Related Research Areas

Notch Pathway

Citations for Human Jagged 2 Antibody

Customer Reviews for Human Jagged 2 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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  • Human Jagged 2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 02/16/2020
    Human Jagged 2 Antibody AF1726
  • Human Jagged 2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 11/11/2018
    See Therapeutic Antibody Targeting Tumor- and Osteoblastic Niche-Derived Jagged1 Sensitizes Bone Metastasis to Chemotherapy Cancer Cell Volume 32, Issue 6, 11 December 2017, Pages 731-747.e6 For experimental details. Note that antibody cross-reacts with hJag1
    Human Jagged 2 Antibody AF1726
  • Human Jagged 2 Antibody
    Name: simon liu
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Bladder tissue
    Species: Human
    Verified Customer | Posted 12/19/2017
    Human Jagged 2 Antibody AF1726

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Protocols

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