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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1102542
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Product Specifications
Immunogen
E. coli-derived recombinant human Jarid1A/KDM5A
Lys127-Ser287
Accession # P29375
Lys127-Ser287
Accession # P29375
Specificity
Detects recombinant human Jarid1A/KDM5a protein in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Jarid1A Antibody (1102542)
Detection of Human Jarid1A by Western Blot.
Western Blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Jarid1A Monoclonal Antibody (Catalog # MAB11705) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Jarid1A at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human Jarid1A by Simple WesternTM.
Left: Simple Western lane view shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.75 mg/ml. A specific band was detected for Jarid1A at approximately 191 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Mouse Anti-Human Jarid1A Monoclonal Antibody (Catalog # MAB11705) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 66-440kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human Jarid1A Monoclonal Antibody (Catalog # MAB11705) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the NTera‑2 human testicular embryonic carcinoma cell line.Applications for Human Jarid1A Antibody (1102542)
Application
Recommended Usage
Simple Western
2-10 µg/mL
Sample: NTera-2 human testicular embryonic carcinoma cell line
Sample: NTera-2 human testicular embryonic carcinoma cell line
Western Blot
2 µg/mL
Sample: NTera-2 human testicular embryonic carcinoma cell line
Sample: NTera-2 human testicular embryonic carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Jarid1A
References
- Harmeyer KM, Facompre ND, Herlyn M, Basu D. JARID1 Histone Demethylases: Emerging Targets in Cancer. Trends Cancer. 2017 Oct;3(10):713-725. doi: 10.1016/j.trecan.2017.08.004. Epub 2017 Sep 12. PMID: 28958389; PMCID: PMC5679451.
- Yang GJ, Zhu MH, Lu XJ, Liu YJ, Lu JF, Leung CH, Ma DL, Chen J. The emerging role of KDM5A in human cancer. J Hematol Oncol. 2021 Feb 17;14(1):30. doi: 10.1186/s13045-021-01041-1. PMID: 33596982; PMCID: PMC7888121.
Long Name
Lysine-specific demethylase 5A
Alternate Names
EC 1.14.11.67, KDM5A, RBBP-2, RBBP2, RBP2
Gene Symbol
KDM5A
UniProt
Additional Jarid1A Products
Product Documents for Human Jarid1A Antibody (1102542)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Jarid1A Antibody (1102542)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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