Human Kallikrein 6/Neurosin Antibody

R&D Systems | Catalog # AF2008

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Kallikrein 6/Neurosin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 6
Glu17-Lys244
Accession # Q92876

Specificity

Detects human Kallikrein 6 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Kallikrein 3, rhKallikrein 5, rhKallikrein 11, rhKallikrein 13, rhKallikrein 14, and rhKallikrein 15 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Kallikrein 6/Neurosin Antibody

Kallikrein 6/Neurosin antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Kallikrein 6/Neurosin in Human Breast Cancer Tissue.

Kallikrein 6/Neurosin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human Kallikrein 6/Neurosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2008) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Kallikrein 6/Neurosin by Western Blot

Detection of Kallikrein 6/Neurosin by Western Blot

MME and KLK6 proteases are increased in conditioned media in a stress-dependent manner. Protease profiler array membranes (R&D systems) with 35 protease capture antibodies printed in duplicate were probed with CM from (A) PC3 24 h post-stress and (B) 1532CPTX cells 16 h post-stress. Pixel density after immunoblot exposure was used to identify common proteases between the two cell lines that were significantly increased (red/purple boxes) when compared to samples without stress (dark blue/light blue boxes): Kallikrein-related peptidase 6 (KLK6) and Membrane metallo-endopeptidase (MME/CD10). (C,D) Immunoblot assays were used to detect KLK6 and MME −/+ stress in PC3 and 1532CPTX cell fraction and CM n = 3. Statistical significance was determined by unpaired Student’s t-test, * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2073-4409/15/2/166), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Kallikrein 6/Neurosin by Western Blot

Detection of Kallikrein 6/Neurosin by Western Blot

Effect of PQR309 on the expression of c-Myc and its possible target genes. (A) After treating with PQR309 for 48 h in HEC-59 cells, Proteome Profiler Human XL Oncology Array was performed according to the manufacturer protocol. The dot intensities were quantified by ImageJ software. The ratio of cells without treatment was set at 1. (B) After treating with PQR309 in AN3CA and HEC-59 cells for 48 h, protein expressions of endoglin, KLK6, p53, and survivin were determined by Western blot. ImageJ software was used to quantify the band intensities of endoglin, KLK6, p53, and survivin. Data shown are the relative expression standardized by the beta -actin protein level. The ratio of cells without treatment was set at 1. (C) Western blot assay was performed to detect the expressions of hTERT and c-Myc in AN3CA and HEC-59 cells after PQR309 treatment for 48 h. ImageJ software was used to quantify the band intensities of hTERT and c-Myc. Data shown are the relative expression standardized by the beta -actin protein level. The ratio of cells without treatment was set at 1. (D) AN3CA (5 × 103 cells/well of 96-well plate) and HEC-59 (6 × 103 cells/well of 96-well plate) were treated with KJ-Pyr-9 (0, 2.5, 5, 10 and 20 μM), an inhibitor of c-Myc, for 72 h. Cell viability was analyzed by MTT assay. (E) Protein expressions of c-Myc, p53, KLK6, and survivin were determined by Western blot. ImageJ software was used to quantify the band intensities of c-Myc, p53, KLK6, and survivin. Data shown are the relative expression standardized by the beta -actin protein level. The ratio of cells without treatment was set at 1. N.D., not detected. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34831139), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Kallikrein 6/Neurosin by Western Blot

Detection of Kallikrein 6/Neurosin by Western Blot

Effect of PQR309 on the expression of c-Myc and its possible target genes. (A) After treating with PQR309 for 48 h in HEC-59 cells, Proteome Profiler Human XL Oncology Array was performed according to the manufacturer protocol. The dot intensities were quantified by ImageJ software. The ratio of cells without treatment was set at 1. (B) After treating with PQR309 in AN3CA and HEC-59 cells for 48 h, protein expressions of endoglin, KLK6, p53, and survivin were determined by Western blot. ImageJ software was used to quantify the band intensities of endoglin, KLK6, p53, and survivin. Data shown are the relative expression standardized by the beta -actin protein level. The ratio of cells without treatment was set at 1. (C) Western blot assay was performed to detect the expressions of hTERT and c-Myc in AN3CA and HEC-59 cells after PQR309 treatment for 48 h. ImageJ software was used to quantify the band intensities of hTERT and c-Myc. Data shown are the relative expression standardized by the beta -actin protein level. The ratio of cells without treatment was set at 1. (D) AN3CA (5 × 103 cells/well of 96-well plate) and HEC-59 (6 × 103 cells/well of 96-well plate) were treated with KJ-Pyr-9 (0, 2.5, 5, 10 and 20 μM), an inhibitor of c-Myc, for 72 h. Cell viability was analyzed by MTT assay. (E) Protein expressions of c-Myc, p53, KLK6, and survivin were determined by Western blot. ImageJ software was used to quantify the band intensities of c-Myc, p53, KLK6, and survivin. Data shown are the relative expression standardized by the beta -actin protein level. The ratio of cells without treatment was set at 1. N.D., not detected. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34831139), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Kallikrein 6/Neurosin by Western Blot

Detection of Kallikrein 6/Neurosin by Western Blot

Stress-induced KLK6 impacts MMP-2 activity and cellular invasion. (A) Immunoblot showing siRNA knockdown of KLK6 and MME in PC3 cell fraction and conditioned media 72 h post-transfection and 24 h post-stress, n = 3 (B) Gelatin DQ assay measuring MMP-2 activity in CM from siRNA samples −/+ stress. (C) Matrigel drop invasion assays with untreated PC3 cells incubated with CM from siRNA KD plates −/+ stress. Invasion was measured over 4 days. (D) Proliferation assay with untreated PC3 cells incubated with CM from untreated and stressed siRNA samples. A minimum of 2 individual biological replicates were performed for the above experiments, and the results were combined. Statistical significance was determined by Welch’s student t-test. * p < 0.05, ** p < 0.01, ns = non-significant. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2073-4409/15/2/166), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Kallikrein 6/Neurosin by Western Blot

Detection of Kallikrein 6/Neurosin by Western Blot

MME and KLK6 proteases are increased in conditioned media in a stress-dependent manner. Protease profiler array membranes (R&D systems) with 35 protease capture antibodies printed in duplicate were probed with CM from (A) PC3 24 h post-stress and (B) 1532CPTX cells 16 h post-stress. Pixel density after immunoblot exposure was used to identify common proteases between the two cell lines that were significantly increased (red/purple boxes) when compared to samples without stress (dark blue/light blue boxes): Kallikrein-related peptidase 6 (KLK6) and Membrane metallo-endopeptidase (MME/CD10). (C,D) Immunoblot assays were used to detect KLK6 and MME −/+ stress in PC3 and 1532CPTX cell fraction and CM n = 3. Statistical significance was determined by unpaired Student’s t-test, * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2073-4409/15/2/166), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Kallikrein 6/Neurosin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 6/Neurosin (Catalog # 5164-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Kallikrein 6/Neurosin (Catalog # 5164-SE)

Reviewed Applications

Read 2 reviews rated 4 using AF2008 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kallikrein 6/Neurosin

Tissue kallikreins are a family of extracellular serine proteases consisting of 15 members (1, 2). The genes of this family aligned in tandem on chromosome 19q13.4 represent the largest contiguous group of proteases within the human genome. Tissue kallikreins have attracted great interest as potential biomarkers for various cancers, including prostate, ovarian, breast, testicular, and lung (2, 3). Human Kallikrein 6 (hKLK6) is a member of tissue kallikrein family observed in breast and brain tissues, colon carcinoma cells, and oligodedrocytes (1-3). Known protein substrates of hKLK6 are myelin basic protein, the precursor of the A beta amyloid peptide, and plasminogen. Its physiological functions may include the participation in demyelination processes as well as in the progression of inflammatory disease of the CNS. The level of hKLK6 is reduced in brain extracts of Alzheimer patients and increased in serum of patients with ovarian cancer.

References

  1. Emami, N. and E.P. Diamandis (2007) Clin. Chim. Acta 381:78.
  2. Borgoño, C.A. and E.P. Diamandis (2004) Nat. Rev. Cancer 4:876.
  3. Paliouras, M. et al. (2007) Cancer Lett. 249:61.

Alternate Names

KLK6, Neurosin, Protease M, PRSS18, PRSS9, SP59, Zyme

Entrez Gene IDs

5653 (Human)

Gene Symbol

KLK6

UniProt

Q92876

Additional Kallikrein 6/Neurosin Products

Product Documents for Human Kallikrein 6/Neurosin Antibody

Certificate of Analysis

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Product Specific Notices for Human Kallikrein 6/Neurosin Antibody

For research use only

Citations for Human Kallikrein 6/Neurosin Antibody

Customer Reviews for Human Kallikrein 6/Neurosin Antibody (2)

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  • Human Kallikrein 6/Neurosin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell lysate from colon cancer cell line
    Species: Human
    Verified Customer | Posted 04/26/2016
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 20846516
    Species: Human
    Verified Customer | Posted 01/06/2015

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