LCTL (also Lactase-like Protein and Klotho/lactase-phlorizin hydrolase-related protein/KLPH) is a 62 kDa (predicted) member of the glycosyl hydrolase 1 family of proteins. It is expressed in skin and kidney, and may participate in endocrine FGF19 subfamily signaling pathways. Mature human LCTL is a 546 amino acid (aa) type I transmembrane protein that appears to be present in the ER. It contains an apparently nonfunctional glycosidase domain (aa 33‑503), plus a very short five aa cytoplasmic segment that may function as an ER-retention signal. There is one potential alternative start site at Met174. Over aa 23‑541, human LCTL shares 86% aa identity with mouse LCTL.
Human Lactase-like Protein/LCTL Antibody
R&D Systems | Catalog # AF5984
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Lactase-like Protein/LCTL
Arg23-Glu541
Accession # Q6UWM7
Arg23-Glu541
Accession # Q6UWM7
Specificity
Detects human Lactase-like Protein/LCTL in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse Lactase-like Protein/LCTL is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Lactase-like Protein/LCTL Antibody
Detection of Human Lactase-like Protein/LCTL by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Lactase-like Protein/LCTL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5984) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lactase-like Protein/LCTL at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human Lactase-like Protein/LCTL Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Lactase-like Protein/LCTL, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Lactase-like Protein/LCTL, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line
Sample: A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Lactase-like Protein/LCTL
Alternate Names
KLG, KLPH, Lactase like Protein, LCTL
Gene Symbol
LCTL
UniProt
Additional Lactase-like Protein/LCTL Products
Product Documents for Human Lactase-like Protein/LCTL Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Lactase-like Protein/LCTL Antibody
For research use only
Related Research Areas
Citations for Human Lactase-like Protein/LCTL Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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