Human Laminin alpha 4 Antibody (839084)

R&D Systems | Catalog # MAB7340

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 839084
View all Laminin alpha 4 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Laminin  alpha 4
Arg826-Ser1816
Accession # Q16363

Specificity

Detects human Laminin  alpha 4 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Laminin alpha 4 or recombinant human Laminin alpha 3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Laminin alpha 4 Antibody (839084)

Laminin a4 antibody in T98G Human Cell Line by Immunocytochemistry (ICC).

Laminin alpha 4 in T98G Human Cell Line.

Laminin a4 was detected in immersion fixed T98G human glioblastoma cells cultured with (upper panel) or without (lower panel) StemXVivo EMT Inducing Media Supplement (Catalog # CCM017) using Mouse Anti-Human Laminin a4 Monoclonal Antibody (Catalog # MAB7340) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Laminin alpha 4 Antibody (839084)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed T98G human glioblastoma cells cultured with EMT Inducing Media Supplement (Catalog # CCM017)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin alpha 4

Laminin alpha 4 (LAMA-4 [Laminin subunit alpha 4]; also Laminin-14/423, -8/411 and -9/421 subunit alpha) is a 180-230 kDa secreted member of the laminin family of molecules. It is found in the basement membranes of adipocytes, endothelial cells, cardiac and visceral smooth muscle cells, fibroblasts, keratinocytes, and pancreatic acinar cells. Laminin is a heterotrimeric glycoprotein that attaches cells to basement membranes. It is composed of covalently-linked alpha -, beta - and gamma -chains, and is known to attach cells to basement membranes. In the case of the alpha -subunit, this is mediated through binding to multiple integrins ( alpha 3 beta 1, alpha v beta 3 and alpha 6 beta 1), plus dystroglycan and the syndecans-2 and -4. Mature human Laminin alpha 4 is 1799 amino acids (aa) in length (aa 25-1823). It contains multiple domains, including four EGF‑like domains (aa 82-255) and five Laminin G-like domains (aa 833-1820). There is a chondroitin sulfate attachment at the N‑terminus that accounts for 20-30 kDa of MW. Laminin alpha 4 is cleaved between the Laminin G-like 3 and 4 domains, creating a soluble 44 kDa fragment that possesses antibacterial activity. There are two potential splice variants, one that shows a deletion of aa 266-272, and another that contains a 55 aa substitution for aa 66-1823. Over aa 826-1816, human Laminin alpha 4 shares 91% aa sequence identity with mouse Laminin alpha 4.

Alternate Names

LAMA4

Entrez Gene IDs

3910 (Human); 16775 (Mouse); 309816 (Rat)

Gene Symbol

LAMA4

UniProt

Q16363

Additional Laminin alpha 4 Products

Product Documents for Human Laminin alpha 4 Antibody (839084)

Certificate of Analysis

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Product Specific Notices for Human Laminin alpha 4 Antibody (839084)

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Human Laminin alpha 4 Antibody (839084)

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