Human Langerin/CD207 Antibody

Langerin/CD207 in Human Skin.

Langerin/CD207 was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human Langerin/CD207 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2088) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of Langerhans cells in the skin epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Langerin/CD207 by Flow Cytometry

Definition of human dermal/lamina propria mononuclear phagocytes by flow cytometry.A Following collagenase digestion six distinct CD3−CD19−CD45+HLA-DR+ mononuclear phagocyte subsets from human abdominal skin were defined, (i) tissue resident macrophages (green) were defined as autofluorescent+CD14+, (ii) type 1 conventional dendritic cells (cDC1; purple) were defined as autofluorescent−, XCR1+, CD14−, type 2 conventional dendritic cells (cDC2; blue) were defined as autofluorescent−, XCR1−, CD14−, CD1c+, and could be split into two populations, (iii) a langerin− population (light blue) and (iv) a langerin+ population (dark blue), (v) CD14+CD1c− cells (red) were defined as autofluorescent−, XCR1−, CD14+, CD1c−, (vi) CD14+CD1c+ cells (orange) were defined as autofluorescent−, XCR1−, CD14+, CD1c+. Representative plot of n = 52 abdominal donors is shown. B Relative proportions of each subset of mononuclear phagocyte as a percentage of CD45+ HLA-DR+ gate across the human anogenital/colorectal tracts were determined and mean ± standard deviation plotted. Statistics for subsets in each tissue were generated using the Kruskal–Wallis test: two-tailed Dunn’s multiple comparisons, comparing against abdominal skin tissue. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (abdominal tissue (Abdo) = 52, labia = 8, glans penis = 8, perineum = 3, outer foreskin = 7, inner foreskin = 5, vagina = 6, fossa navicularis (FN) = 8, anal canal = 5, ectocervix = 4, endocervix = 3, penile urethra = 8, rectum = 7, colon = 5). Underneath, a pie chart for each tissue shows the mean proportion of each dermal/lamina propria subset across the human anogenital/colorectal tracts. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33846309), licensed under a CC-BY license. Not internally tested by R&D Systems.