Human LAP (TGF-beta 1) Antibody (27240) [Biotin]

R&D Systems | Catalog # BAM2462

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

Flow Cytometry, Bioassay, ELISA Detection

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # 27240
View all LAP (TGF-beta 1) Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Leu30-Arg278 (Cys33Ser)
Accession # P01137

Specificity

Recognizes human LAP (TGF-beta 1) and human latent TGF-beta 1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human LAP (TGF-beta 1) Antibody (27240) [Biotin]

Human LAP (TGF-beta 1) ELISA Standard Curve

Human LAP (TGF-beta 1) ELISA Standard Curve

Recombinant Human LAP (TGF-beta 1) (Catalog # 246-LP) was serially diluted and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB2461) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human LAP (TGF-beta 1) Antibody (27240) [Biotin]

Human LAP TGF-beta 1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human LAP (TGF-beta 1) Antibody (Catalog # MAB2461)
  • Standard: Recombinant Human LAP (TGF-beta 1) Protein (Catalog # 246-LP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using BAM2462 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAP (TGF-beta 1)

The TGF-beta family includes several related proteins (70 - 80% sequence homology) from mammalian, avain or Xenopus systems that are now designated TGF-beta 1, TGF-beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4 and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF-beta associated non-covalently with LAP. These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Long Name

Latency-associated Peptide

Alternate Names

LAP (TGFbeta 1)

Entrez Gene IDs

7040 (Human)

Gene Symbol

TGFB1

UniProt

P01137 (Human)

Additional LAP (TGF-beta 1) Products

Product Documents for Human LAP (TGF-beta 1) Antibody (27240) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LAP (TGF-beta 1) Antibody (27240) [Biotin]

For research use only

Citations for Human LAP (TGF-beta 1) Antibody (27240) [Biotin]

Customer Reviews for Human LAP (TGF-beta 1) Antibody (27240) [Biotin] (2)

3.5 out of 5
2 Customer Ratings
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Customer Images

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  • Human LAP TGF-beta 1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human and Mouse
    Verified Customer | Posted 03/29/2019
    I tested the antibody using direct ELISA to see if it cross reacted with mouse latent TGF beta or mature mouse TGF beta 1 recombinant proteins.
    Human LAP (TGF-beta 1) Antibody (27240) [Biotin] BAM2462
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 22939403
    Species: Mouse
    Verified Customer | Posted 01/16/2015

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