Human LAR Antibody (384727)

R&D Systems | Catalog # MAB3004

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 384727
View all LAR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAR
Ala27-Glu1251
Accession # NP_569707

Specificity

Detects endogenous human LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular domain (ECD). It does not cross-react with recombinant human PTPRM, PTPRK, DEP1, or PTPR gamma.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Human LAR Antibody (384727)

Detection of Human LAR antibody by Western Blot.

Detection of Human LAR by Western Blot.

Western blot shows lysates of CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human LAR Monoclonal Antibody (Catalog # MAB3004) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LAR at approximately 139 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human LAR Antibody (384727)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: CHP-100 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAR

Leukocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phosphatase, Receptor-type F (PTPRF), is an integral membrane protein with anon-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium.

Long Name

Leukocyte Antigen-Related Tyrosine Phosphatase

Alternate Names

PTPRF

Entrez Gene IDs

5792 (Human); 104121 (Mouse)

Gene Symbol

PTPRF

UniProt

NP_569707

Additional LAR Products

Product Documents for Human LAR Antibody (384727)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LAR Antibody (384727)

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

Citations for Human LAR Antibody (384727)

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