Human Latent TGF-beta bp2/LTBP-2 Antibody

R&D Systems | Catalog # AF3850

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Latent TGF-beta bp2/LTBP-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Latent TGF‑ beta  bp2/LTBP‑2
Gln36-Glu1821
Accession # Q14767

Specificity

Detects human Latent TGF‑ beta  bp2/LTBP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) LTBP-2 is observed, and less than 1% cross-reactivity with recombinant human LTBP-1 and rmLTBP-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Latent TGF-beta bp2/LTBP-2 Antibody

Detection of Human Latent TGF-beta bp2/LTBP-2 antibody by Western Blot.

Detection of Human Latent TGF‑ beta bp2/LTBP‑2 by Western Blot.

Western blot shows lysates of HFL1 human fetal lung fibroblast cell line and human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Latent TGF-beta bp2/LTBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3850) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Latent TGF-beta bp2/LTBP-2 at approximately 210-255 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Latent TGF-beta bp2/LTBP-2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HFL1 human fetal lung fibroblast cell line and human heart tissue

Reviewed Applications

Read 1 review rated 5 using AF3850 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Latent TGF-beta bp2/LTBP-2

LTBP-2 (Latent TGF-beta Binding Protein 2; also C14orf141) is a 220-250 kDa secreted glycoprotein member of the LTBP family of proteins. In SDS-PAGE and under reducing conditions, LTBP-2 runs at 220-250 kDa; under nonreducing conditions, LTBP-2 runs between 205-220 kDa. Although it is described as being a TGF-beta binding protein, it does not possess the ability to bind covalently to TGF-beta. Instead, it appears to bind to heparin sulfate moieties on syndecan-4 and perlecan, plus fibrillin-1 and fibulin-5, suggesting a role in both elastic fiber formation and the maintenance of basement membrane integrity. In addition, LTPB-2 is now known to form a complex with PC5/6A intracellularly, and upon secretion, dampen PC5 activation in the extracellular environment. Cells known to express LTBP-2 include lens and nonpigmented ciliary epithelium, fibroblasts, vascular smooth muscle and chondrocytes. Mature human LTBP-2 is 1786 amino acids (aa) in length (aa 36-1821). It possesses three heparin-binding regions (aa 94-115, 232-249, and 344-354), plus 20 EGF-like domains (aa 187-1818) that contain four interspersed TGF-beta type binding segments. Over aa 36-1821, human LTBP-2 shares 80% aa sequence identity with mouse LTBP-2.

Alternate Names

Latent TGFbeta bp2, LTBP-2, LTBP2

Entrez Gene IDs

4053 (Human)

Gene Symbol

LTBP2

UniProt

Q14767

Additional Latent TGF-beta bp2/LTBP-2 Products

Product Documents for Human Latent TGF-beta bp2/LTBP-2 Antibody

Certificate of Analysis

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Product Specific Notices for Human Latent TGF-beta bp2/LTBP-2 Antibody

For research use only

Citations for Human Latent TGF-beta bp2/LTBP-2 Antibody

Customer Reviews for Human Latent TGF-beta bp2/LTBP-2 Antibody (1)

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  • Human Latent TGF-beta  bp2/LTBP-2 Antibody
    Name: Andrei Turtoi
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Liver metastasis from colorectal tumor
    Species: Human
    Verified Customer | Posted 01/24/2017
    - Antigen retrieval: Heat induced/Citrate buffer pH 6 - Primary antibody dilution: 1:50
    Human Latent TGF-beta bp2/LTBP-2 Antibody AF3850

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