LILRA4, also known as ILT7 and CD85g, is a 499 amino acid (aa) type I transmembrane glycoprotein that contains four Ig-like domains in its extracellular region. LILRA4 is selectively expressed on plasmacytoid dendritic cells (pDC). It binds to BST2/CD317 on bone marrow stromal cells and associates with the gamma subunit of Fc epsilon RI. This receptor complex transmits signals that inhibit the TLR-induced production of type I interferon. The LILRA4-mediated interactions between pDC and tumor cells similarly inhibit the production of proinflammatory cytokines and contribute to reduced anti-tumor immune responses. Alternate splicing of human LILRA4 generates an isoform that lacks the N-terminal 43 aa of the extracellular domain.
Human LILRA4/CD85g/ILT7 Antibody (656688)
R&D Systems | Catalog # MAB6287
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 656688
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRA4/CD85g/ILT7
Glu24-Asn446
Accession # P59901
Glu24-Asn446
Accession # P59901
Specificity
Detects human LILRA4/CD85g/ILT7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) LILRA5, rhILT2, 3, 4, 5, or 6 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human LILRA4/CD85g/ILT7 Antibody (656688)
Detection of LILRA4/CD85g/ILT7 in Human PBMCs by Flow Cytometry.
Human peripheral blood monocytes were labeled with Mouse Anti-Human LILRA4/CD85g/ILT7 Monoclonal Antibody (Catalog # MAB6287) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B) and Human DLEC/CLEC4C/BDCA-2 Fluorescein-conjugated Polyclonal Antibody (Catalog # FAB1376F). Quadrant markers were set based on control antibody staining (Catalog # MAB003).Applications for Human LILRA4/CD85g/ILT7 Antibody (656688)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LILRA4/CD85g/ILT7
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 4
Alternate Names
CD85g, ILT7
Entrez Gene IDs
23547 (Human)
Gene Symbol
LILRA4
UniProt
Additional LILRA4/CD85g/ILT7 Products
Product Documents for Human LILRA4/CD85g/ILT7 Antibody (656688)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human LILRA4/CD85g/ILT7 Antibody (656688)
For research use only
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