Human Lipin 1 Antibody

R&D Systems | Catalog # AF3885

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Lipin 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Lipin 1
Asp448-Leu574
Accession # Q14693

Specificity

Detects human Lipin 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human (rh) Lipin 2 and rhLipin 3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Lipin 1 Antibody

Lipin 1 antibody in Human Adipocytes by Immunocytochemistry (ICC).

Lipin 1 in Human Adipocytes.

Lipin 1 was detected in immersion fixed human mesenchymal stem cell-derived adipocytes using Goat Anti-Human Lipin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Lipin 1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human mesenchymal stem cell-derived adipocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human Lipin 1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lipin 1

Lipin 1 is a 100 kDa phosphatidate phosphatase. Mammalian Lipin 1 has been identified as an enzyme that regulates fat metabolism. There is one human Lipin-1. In mice, two Lipin-1 isoforms have been reported, alpha and beta. Lipin 1 alpha is a nuclear protein and is 96% and 98 % identical to human Lipin 1 and rat Lipin 1 alpha, respectively. Lipin 1 beta is cytoplasmic.

Long Name

Phosphatidate Phosphatase LPIN1

Alternate Names

LPIN1

Entrez Gene IDs

23175 (Human); 14245 (Mouse); 313977 (Rat)

Gene Symbol

LPIN1

UniProt

Q14693

Additional Lipin 1 Products

Product Documents for Human Lipin 1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Lipin 1 Antibody

For research use only

Related Research Areas

Obesity
Phosphatases and Regulators

Citations for Human Lipin 1 Antibody

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Protocols

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FAQs

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Associated Pathways

mTOR Signaling Pathway
mTOR Signaling Pathway Thumbnail