MAP3K8, also called Tpl2 (tumor progression locus 2) or COT (Cancer Osaka Thyroid oncogene) is a cytosolic Ser/Thr kinase. MAP3K8 is one of the MAP kinase kinase (MAPKK) kinases that regulates the ERK1/ERK2 pathway in response to IL-1, facilitating IL-8 and MIP-1 beta production. MAP3K8 activates I kappa B kinases and induces the nuclear production of NF-kappa B. Human MAP3K8 shares 97% and 98% amino acid (aa) identity with mouse and rat MAP3K8, respectively, within the region used as an immunogen. Alternative start sites can give a 58 kDa (467 aa) isoform with stronger activity but shorter half-life than a 52 kDa (397 aa) isoform.
Human MAP3K8/Tpl2/COT Antibody (634130)
R&D Systems | Catalog # MAB4586
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 634130
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Product Specifications
Immunogen
E. coli-derived recombinant human MAP3K8/Tpl2/COT
Gln173-Gln395
Accession # P41279
Gln173-Gln395
Accession # P41279
Specificity
Detects human MAP3K8/Tpl2/COT in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MAP3K8/Tpl2/COT Antibody (634130)
Detection of Human MAP3K8/Tpl2/COT by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MO7e human megakaryocytic leukemic cell line. PVDF Membrane was probed with 1 µg/mL of Human MAP3K8/Tpl2/COT Monoclonal Antibody (Catalog # MAB4586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for MAP3K8/Tpl2/COT at approximately 58 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human MAP3K8/Tpl2/COT Antibody (634130)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MO7e human megakaryocytic leukemic cell line
Sample: Jurkat human acute T cell leukemia cell line and MO7e human megakaryocytic leukemic cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MAP3K8/Tpl2/COT
Long Name
Mitogen-activated Protein Kinase Kinase Kinase 8
Alternate Names
COT, EST, ESTF, MAP3K8, Tpl-2
Gene Symbol
MAP3K8
UniProt
Additional MAP3K8/Tpl2/COT Products
Product Documents for Human MAP3K8/Tpl2/COT Antibody (634130)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MAP3K8/Tpl2/COT Antibody (634130)
For research use only
Related Research Areas
Citations for Human MAP3K8/Tpl2/COT Antibody (634130)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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