The human METAP2 gene encodes Methionine Aminopeptidase 2, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). METAP2 plays an important role in the development of different types of cancer (3) and has been a novel target for developing anti-cancer drugs.
Human Methionine Aminopeptidase 2/METAP2 Antibody
R&D Systems | Catalog # AF3795
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Porcine
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminopeptidase 2/METAP2
Ala2-Tyr478
Accession # P50579
Ala2-Tyr478
Accession # P50579
Specificity
Detects human Methionine Aminopeptidase 2/METAP2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Methionine Aminopeptidase 2/METAP2 Antibody
Detection of Human Methionine Aminopeptidase 2 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, LS180 human colorectal adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Methionine Aminopeptidase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3795) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Methionine Aminopeptidase 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human Methionine Aminopeptidase 2/METAP2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 2/METAP2 (Catalog # 3795-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 2/METAP2 (Catalog # 3795-ZN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line, LS180 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line
Sample: COLO 205 human colorectal adenocarcinoma cell line, LS180 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Methionine Aminopeptidase 2/METAP2
References
- Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta 1477:157.
- Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
- Selvakumar, P. et al. (2006) Biochim. Biophys. Acta 1765:148.
Alternate Names
Amp2, METAP2, MNPEP, p67eIF2
Gene Symbol
METAP2
UniProt
Additional Methionine Aminopeptidase 2/METAP2 Products
Product Documents for Human Methionine Aminopeptidase 2/METAP2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Methionine Aminopeptidase 2/METAP2 Antibody
For research use only
Related Research Areas
Citations for Human Methionine Aminopeptidase 2/METAP2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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