Human MMP-19 Antibody

R&D Systems | Catalog # AF6790

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMP-19 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-19
Leu229-Tyr508
Accession # Q99542

Specificity

Detects human MMP-19 in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMP-19 Antibody

Detection of Human MMP-19 antibody by Western Blot.

Detection of Human MMP-19 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for MMP-19 at approximately 57 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MMP-19 antibody in Human Melanoma by Immunohistochemistry (IHC-P).

MMP-19 in Human Melanoma.

MMP-19 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human MMP-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6790) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of MMP-19 by Western Blot

Detection of MMP-19 by Western Blot

MMP-19 and MMP-20 high expressions induced anti-cancer drug resistance. (a) MMP-19 and MMP-20 were assayed by Western Blotting in indicated ovarian carcinoma cell lines. The numbers listed below the blots indicate the optical density ratio of target protein to beta -actin. (b–g) Direct comparison of cell death and viabilities of ovarian cancer cell lines with low (Ovcar8) and high (Cov362) MMP-20 expressions after the treatment of A-1210477 (b,d,f) and Vincristine (c,e,g). After the cell lines were treated with indicated drugs for 48 hours, the percentages of pre-G1 cells (b,c), Annexin V+ or PI+ cells (d,e), or the ratio of OD450 by WST-8 (f,g) were assayed. (h,i) Direct comparison of cell death and viabilities of ovarian cancer cell lines with low (Ovcar5) and high (Ho8910) MMP-19 expressions after the treatment of A-1210477 (h) and Vincristine (i). After the cell lines were treated with indicated drugs for 48 hours, the percentages of pre-G1 cells were measured. Error bars, mean ± S.D. of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31406154), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMP-19 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and human small intestine tissue

Reviewed Applications

Read 2 reviews rated 4 using AF6790 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-19

MMP-19 (Matrix metalloprotease 19; also MMP-18 and MMP RASI) is a 55-59 kDa member of the peptidase M10A family of enzymes. It is widely expressed, being secreted by stratum basale keratinocytes, smooth muscle cells, epiphysial cartilage chondrocytes and monocytes/macrophages. MMP-19 has multiple substrates, including components of the basement membrane (type IV collagen; laminin; nidogen), fibronectin, aggrecan plus COMP, and IGFBP3, this latter cleavage resulting in the release of active IGF-I. Studies involving MMP-19 demonstrate an antiangiogenic function. This is attributable to the processing of plasminogen, generating angiostatin-like molecules, and the creation of an environment that promotes the ECM retention of soluble VEGF. Human proMMP-19 is 490 amino acids (aa) in length. It contains an autocleavable 9 kDa propeptide (aa 19-97) plus a 411 aa mature region (aa 98-508). The mature region contains a Zn catalytic region (aa 103-256) plus four hemopexin-like domains (aa 293-508). There are three additional potential isoforms. One shows an 88 aa substitution for aa 300-508, a second contains a 12 aa substitution for aa 1-298, while a third possesses an alternative start site at Met80. Over aa 229-508, human MMP-19 shares 76% aa identity with mouse MMP-19.

Long Name

Matrix Metalloproteinase 19

Alternate Names

MMP-18, MMP19, RASI-1

Entrez Gene IDs

4327 (Human); 58223 (Mouse); 304608 (Rat)

Gene Symbol

MMP19

UniProt

Q99542 (Human)

Additional MMP-19 Products

Product Documents for Human MMP-19 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP-19 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human MMP-19 Antibody

Customer Reviews for Human MMP-19 Antibody (2)

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  • Human MMP-19 Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Cell culture supernatant and Plasma
    Species: Human
    Verified Customer | Posted 04/21/2017
    in comparison: Animal models have become increasingly useful to examine the potential of MMP inhibitors to suppress aneurysm development and growth in vivo
    Human MMP-19 Antibody AF6790
  • Human MMP-19 Antibody
    Name: Ruhul Choudhury
    Application: Immunohistochemistry
    Sample Tested: Human First trimester decidua
    Species: Goat
    Verified Customer | Posted 01/23/2017
    sodium citrate antigen retrieval
    Human MMP-19 Antibody AF6790

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