Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 101724
View all MMP-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑2
Ala30-Cys660
Accession # P08253

Specificity

Detects the pro and active forms of human MMP-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MMP-1, -3, -7, -8, -9, -10, -12, or -13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MMP-2 Antibody (101724)

Detection of MMP-2 by Western Blot

Detection of MMP-2 by Western Blot

Abolishment of TGF-beta 1-mediated matrix remodeling by p38 and JNK inhibitors in the primary cultured orbital fibroblasts from patients with Graves’ ophthalmopathy (GO). (A) Orbital fibroblasts from GO patients were preincubated with the p38 inhibitor SB202190 (20 μM) and the JNK inhibitor SP600125 (20 μM), respectively, for 1 h, followed by TGF-beta 1 (5 ng/mL) treatment for another 24 h. Then, the expression levels of TIMP-1, TIMP-3, MMP-2, and MMP-9 were analyzed by Western blots; (B) The relative intensities of TIMP-1, TIMP-3, MMP-2, and MMP-9 expression normalized to each GAPDH control and DMSO control without TGF-beta 1 treatment were defined as 1.0. Then, the other relative intensity (folds) was presented. By three independent Western blot experiments, together data from the same patient strain were averaged. Then, the means of different patient (GO1–GO4, n = 4) strains were averaged; (C) The enzyme activities of MMP-2/-9 from the cultured medium were determined. The representative histogram was plotted based on the mean values of relative fluorescence units from the primary cultured orbital fibroblasts from the four GO patients. Data were presented as means ± S.D. of the results from three independent experiments. * p < 0.05 and ** p < 0.01 vs. control without TGF-beta 1 treatment; # p < 0.05 and ## p < 0.01 vs. TGF-beta 1 treatment. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33799469), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMP-2 Antibody (101724)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑2 (Catalog # 902-MP), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025)

Reviewed Applications

Read 2 reviews rated 5 using MAB9022 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-2

MMP-2, also called gelatinase A, is a matrix metalloproteinase that can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. MMP-2 is produced by neutrophils, macrophages and monocytes.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Gene Symbol

MMP2

UniProt

P08253

Additional MMP-2 Products

Product Documents for Human MMP-2 Antibody (101724)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP-2 Antibody (101724)

For research use only

Citations for Human MMP-2 Antibody (101724)

Customer Reviews for Human MMP-2 Antibody (101724) (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human MMP-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HepG2 human hepatocellular carcinoma cell line and MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 07/23/2018
    Total cell lysates from HepG2 and MDA-MB-231 were subjected to western blot. PVDF membrane were probed with 1 um/ml Human MMP-2 Antibody (MAB9022). A specific band was detected for MMP2 at approximately 70 kDa. This experiment was conducted under reducing conditions
    Human MMP-2 Antibody (101724) MAB9022
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Purified Human MMP2, Purified MMP9 and Purified Human MMP9
    Species: Human
    Verified Customer | Posted 07/02/2018
    Does not cross-react with human MMP9
    Human MMP-2 Antibody (101724) MAB9022

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