Apolipoprotein A-IV/ApoA4 Antibody

R&D Systems | Catalog # AF8125

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Apolipoprotein A-IV/ApoA4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-dervied recombinant human Apolipoprotein A-IV/ApoA
Glu21-Ser396
Accession # P06727

Specificity

Detects human Apolipoprotein A-IV/ApoA in direct ELISAs. Detects human and mouse in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Apolipoprotein A-IV/ApoA4 Antibody

Detection of Human Apolipoprotein A-IV/ApoA4 antibody by Western Blot.

Detection of Human Apolipoprotein A-IV/ApoA4 by Western Blot.

Western blot shows human plasma and human serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Apolipoprotein A-IV/ApoA4 antibody by Western Blot.

Detection of Mouse Apolipoprotein A-IV/ApoA4 by Western Blot.

Western blot shows mouse plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Apolipoprotein A-IV/ApoA4 antibody in Human Small Intestine by Immunohistochemistry (IHC-P).

Apolipoprotein A-IV/ApoA4 in Human Small Intestine.

Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Apolipoprotein A-IV/ApoA4 antibody in Human Ileum and Descending Colon by Immunohistochemistry (IHC-P).

Apolipoprotein A-IV/ApoA4 in Human Ileum and Descending Colon.

Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human ileum (left panel) and descending colon (right panel) using Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue).
Detection of Human Apolipoprotein A-IV/ApoA4 antibody by Simple WesternTM.

Detection of Human Apolipoprotein A‑IV/ApoA4 by Simple WesternTM.

Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 51 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Apolipoprotein A-IV/ApoA4 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small intestine and human descending colon

Simple Western

2 µg/mL
Sample: Human Plasma

Western Blot

0.2-2 µg/mL
Sample: Human plasma, human serum, and mouse plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein A-IV/ApoA4

Apolipoprotein A4 (ApoA4) is a 45 kDa glycoprotein of the lipid transport system.  Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption.  Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis.  ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well.  Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 (aa 21-396) shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

Alternate Names

Apolipoprotein AIV

Entrez Gene IDs

337 (Human); 11808 (Mouse); 25080 (Rat)

Gene Symbol

APOA4

UniProt

P06727

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Apolipoprotein A-IV/ApoA4 Antibody

Certificate of Analysis

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Product Specific Notices for Apolipoprotein A-IV/ApoA4 Antibody

For research use only

Related Research Areas

Obesity

Citations for Apolipoprotein A-IV/ApoA4 Antibody

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Protocols

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