Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as anutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA14 is a type I membrane enzyme highly expressed in all parts of the central nervous system with lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder, and skeletal muscle (2).
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XIV/CA14
Gly19-Met290
Accession # Q9ULX7
Gly19-Met290
Accession # Q9ULX7
Specificity
Detects human Carbonic Anhydrase XIV/CA14 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CA1, rhCA2, rhCA3, rhCA4, rhCA8, rhCA9, rhCA10, rhCA12, and rhCA13 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Carbonic Anhydrase XIV/CA14 Antibody
Detection of Human and Mouse Carbonic Anhydrase XIV/CA14 by Western Blot.
Western blot shows lysates of human and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase XIV/CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2195) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Carbonic Anhydrase XIV/CA14 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase XIV (Catalog # 2195-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase XIV (Catalog # 2195-CA), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Human and mouse brain (cerebellum) tissue
Sample: Human and mouse brain (cerebellum) tissue
Reviewed Applications
Read 1 review rated 5 using AF2195 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Carbonic Anhydrase XIV/CA14
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Fujikawa-Adachi, K. et al. (1999) Genomics 61:74.
Alternate Names
CA14
Gene Symbol
CA14
UniProt
Additional Carbonic Anhydrase XIV/CA14 Products
Product Documents for Carbonic Anhydrase XIV/CA14 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Carbonic Anhydrase XIV/CA14 Antibody
For research use only
Customer Reviews for Carbonic Anhydrase XIV/CA14 Antibody (1)
5 out of 5
1 Customer Rating
Have you used Carbonic Anhydrase XIV/CA14 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Western BlotSample Tested: melanoma cell lysates of human and mouseSpecies: human and mouseVerified Customer | Posted 04/21/2026Whole cell lysates were blotted with 1o CA14 goat polyclonal antibody AF2195 and then 2o anti-Goat HRP. The results are clean without any non-specific bands.
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...