FoxP2 Antibody

R&D Systems | Catalog # AF5647

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FoxP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxP2 isoform 1
Ala640-Glu715
Accession # O15409

Specificity

Detects human and mouse FoxP2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for FoxP2 Antibody

Detection of Human FoxP2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A172 human glioblastoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for FoxP2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of FoxP2 by Immunohistochemistry

Detection of FoxP2 by Immunohistochemistry

Near the LC, Nps colocalizes with Foxp2 and is mutually exclusive with both Calca and Pdyn. (a–c) Neurons that contain Nps mRNA (red) are mutually exclusive with and medial to neurons in the LC, which contain Calca mRNA (magenta). Ubc mRNA (green) is shown for neuroanatomical background. (d–f) A subset of Foxp2‐expressing neurons (blue) also express Nps (red) medial to the LC. (g–k) This Nps‐expressing subset is mutually exclusive with Pdyn mRNA (yellow). Scale bars are 100 μm (a–c), 50 μm (d–f), 20 μm (g–k) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36036349), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FoxP2 by Immunohistochemistry

Detection of FoxP2 by Immunohistochemistry

Dlx1+ arkypallidal GABAergic projection neurons, but not prototypical neurons, are increased in the PND13 BACHD Globus Pallidus. (A/A’–D/D′) Representative immunofluorescence images of the Globus Pallidus from PND13 mice stained for the prototypical neuron marker, PV+ cells (A/A’), the arkypallidal neuron marker, Foxp2 (B/B′), EGFP driven by the Dlx1 promoter (C/C′), and combined Foxp2/Dlx1 double labeling (D/D′). (E–H) Quantification of PV+ prototypical (E), all Foxp2+ arkypallidal cells (F), Foxp2+/Dlx1+ arkypallidal cells (G), and Foxp2+/Dlx1− cells (H). Data are presented as box-and-whisker plots showing median, interquartile range, and minimum/maximum values. Individual data points represent biological replicates. Statistical comparisons were performed using unpaired Student’s t-test; p < 0.05 was considered significant. Scale bars: 200 μm (A, D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41654201), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FoxP2 by Immunohistochemistry

Detection of FoxP2 by Immunohistochemistry

The caudal cluster of Nps Cre‐reporter neurons is medial to the LC, with some neurons extending into Barrington's nucleus (Bar). Most L10GFP‐expressing neurons (green, b) in this region contained FoxP2 (blue, c) and not Lmx1b (red, a). FoxP2 and Lmx1b identified mutually exclusive neuronal populations, with FoxP2‐immunoreactive neurons encircling Bar and light Lmx1b immunoreactivity identifying neurons in the LC (f). (d–g) Merged images. Bar contained several L10GFP‐expressing neurons that lacked FoxP2. Scale bars in (a; also applies to b–f) and (g) are 200 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36036349), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FoxP2 by Immunohistochemistry

Detection of FoxP2 by Immunohistochemistry

Dlx1+ arkypallidal GABAergic projection neurons, but not prototypical neurons, are increased in the PND13 BACHD Globus Pallidus. (A/A’–D/D′) Representative immunofluorescence images of the Globus Pallidus from PND13 mice stained for the prototypical neuron marker, PV+ cells (A/A’), the arkypallidal neuron marker, Foxp2 (B/B′), EGFP driven by the Dlx1 promoter (C/C′), and combined Foxp2/Dlx1 double labeling (D/D′). (E–H) Quantification of PV+ prototypical (E), all Foxp2+ arkypallidal cells (F), Foxp2+/Dlx1+ arkypallidal cells (G), and Foxp2+/Dlx1− cells (H). Data are presented as box-and-whisker plots showing median, interquartile range, and minimum/maximum values. Individual data points represent biological replicates. Statistical comparisons were performed using unpaired Student’s t-test; p < 0.05 was considered significant. Scale bars: 200 μm (A, D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41654201), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FoxP2 by Immunohistochemistry

Detection of FoxP2 by Immunohistochemistry

In the rostral lateral PB, neurons expressing the L10GFP Cre‐reporter for Nps contain FoxP2 but not Lmx1b. In this region, immunoreactivity for Lmx1b (red, a) and FoxP2 (blue, c) define mutually exclusive populations of neurons except in the KF, which contains neurons coexpressing both transcription factors (e). L10GFP‐expressing neurons (green, b) are dorsal to Lmx1b‐immunolabeled neurons (d) and contain FoxP2 immunolabeling (f). These L10GFP‐expressing neurons are dorsal to the KF in the lateral PB (g). Scale bars in (f; also applies to a–e) and (g) are 500 μm. Additional mcp, middle cerebellar peduncle; DR, dorsal raphe nucleus Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36036349), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FoxP2 by Immunohistochemistry

Detection of FoxP2 by Immunohistochemistry

The E12.5 BACHD subpallium displays selective alterations of GABAergic precursor cells. Representative immunofluorescence images of the lateral ganglionic eminence [LGE] and ventral pallium [VP] (A–D, A’–D′), the primordium of the globus pallidus (GP; F–G, F′–G’), and the medial ganglionic eminence (MGE) germinative zone (H/H′). Tissue sections were immunostained for Lhx6 (A/A′), Satb1 (B/B′), EGFP driven by the Dlx1 promoter (C/C′), merged staining in A to C (D/D′), Lhx6/Dlx1 (E/E′), Nkx2–1/Dlx1 (F/F′), Lhx6/Foxp2/Dlx1 (G/G′), and VZ/SVZ Nkx2–1/Dlx1 (H/H′). Quantification analyses included LGE mantle: Lhx6+ (I), Satb1+/Lhx6+ (J), and Mafb+ (K); VP: EGFP+ driven by the Dlx1 promoter (L), Lhx6+ cells (M), Satb1+/Lhx6+ (N), and Mafb+ (O); Globus Pallidus (GP): Nkx2–1+ (P) and Foxp2+/Lhx6+ (Q) cells; and MGE VZ/SVZ Nkx2–1+ progenitors (R). Data are presented as box-and-whisker plots showing the median, interquartile range, and minimum/maximum values. Individual data points represent biological replicates. Statistical comparisons were performed using Mann-Whitney U test (I,J,Q) and unpaired Student’s t-test (K, L-P, R); p < 0.05 was considered significant. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41654201), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FoxP2 by Immunohistochemistry

Detection of FoxP2 by Immunohistochemistry

The caudal cluster of Nps Cre‐reporter neurons is medial to the LC, with some neurons extending into Barrington's nucleus (Bar). Most L10GFP‐expressing neurons (green, b) in this region contained FoxP2 (blue, c) and not Lmx1b (red, a). FoxP2 and Lmx1b identified mutually exclusive neuronal populations, with FoxP2‐immunoreactive neurons encircling Bar and light Lmx1b immunoreactivity identifying neurons in the LC (f). (d–g) Merged images. Bar contained several L10GFP‐expressing neurons that lacked FoxP2. Scale bars in (a; also applies to b–f) and (g) are 200 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36036349), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FoxP2 by Immunohistochemistry

Detection of FoxP2 by Immunohistochemistry

The caudal cluster of Nps Cre‐reporter neurons is medial to the LC, with some neurons extending into Barrington's nucleus (Bar). Most L10GFP‐expressing neurons (green, b) in this region contained FoxP2 (blue, c) and not Lmx1b (red, a). FoxP2 and Lmx1b identified mutually exclusive neuronal populations, with FoxP2‐immunoreactive neurons encircling Bar and light Lmx1b immunoreactivity identifying neurons in the LC (f). (d–g) Merged images. Bar contained several L10GFP‐expressing neurons that lacked FoxP2. Scale bars in (a; also applies to b–f) and (g) are 200 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36036349), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FoxP2 by Immunohistochemistry

Detection of FoxP2 by Immunohistochemistry

In the rostral lateral PB, neurons expressing the L10GFP Cre‐reporter for Nps contain FoxP2 but not Lmx1b. In this region, immunoreactivity for Lmx1b (red, a) and FoxP2 (blue, c) define mutually exclusive populations of neurons except in the KF, which contains neurons coexpressing both transcription factors (e). L10GFP‐expressing neurons (green, b) are dorsal to Lmx1b‐immunolabeled neurons (d) and contain FoxP2 immunolabeling (f). These L10GFP‐expressing neurons are dorsal to the KF in the lateral PB (g). Scale bars in (f; also applies to a–e) and (g) are 500 μm. Additional mcp, middle cerebellar peduncle; DR, dorsal raphe nucleus Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36036349), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for FoxP2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A172 human glioblastoma cell line and U937 human histiocytic lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FoxP2

FoxP2 (Forkhead box protein P2; also CAGH44) is an 85‑95 kDa member of the winged helix transcription factor gene family. It is a transcriptional repressor that is expressed principally in gut and lung epithelium, and in neurons involved in speech and language. Human FoxP2 is 715 amino acids (aa) in length. It contains a huge poly-Gln region (aa 123‑231), a C2H2 Zn-finger domain (aa 346‑371), a Leu-zipper segment (aa 388‑409) and a forkhead DNA binding domain (aa 504‑594). FoxP2 both homodimerizes and heterodimerizes with FoxP1 and P4, and binds CTBP1. There are multiple splice variants. There is an alternate start site at Met93, an 11 aa substitution for aa 57‑715, a 25 aa insert after Gln86, an 11 aa substitution for aa 133‑715, and a ten aa substitution for aa 423‑715. Over aa 587‑715, human and mouse FoxP2 are (100%) identical in aa sequence.

Long Name

Forkhead Box P2

Alternate Names

CAGH44, SPCH1, TNRC10

Entrez Gene IDs

93986 (Human); 114142 (Mouse); 500037 (Rat)

Gene Symbol

FOXP2

UniProt

O15409

Additional FoxP2 Products

Product Documents for FoxP2 Antibody

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Product Specific Notices for FoxP2 Antibody

For research use only

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