HSF4 Antibody (504524)

R&D Systems | Catalog # MAB5695

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 504524
View all HSF4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSF4
Ser309-Pro463
Accession # BAA13433

Specificity

Detects endogenous human and mouse HSF4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HSF4 Antibody (504524)

Detection of Human/Mouse HSF4 antibody by Western Blot.

Detection of Human/Mouse HSF4 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, human LNCaP human prostate cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse HSF4 Monoclonal Antibody (Catalog # MAB5695) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSF4 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for HSF4 Antibody (504524)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, human LNCaP human prostate cancer cell line, and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSF4

HSF4 (heat shock factor 4) is a 53 kDa member of the HSF family of transcription factors. It is highly expressed in the lens where it maintains lens integrity and blocks FGF synthesis. Human HSF4 has two isoforms HSF4B and HSF4A. Human HSF4B is 493 amino acids in length. It contains one DNA binding region (aa 18‑122) and two hydrophobic repeat domains A/B and C (aa 130‑204 and 369‑390). HSF4B activates transcription, whereas, HSF4A represses transcription. HSF4A shows a 45 aa substitution for aa 246‑320. Over aa 309‑463, human HSF4B shares 73% aa sequence identity with mouse HSF4B.

Long Name

Heat Shock Transcription Factor 4

Alternate Names

AT-HSFB1, ATHSF4, CTM, HSFB1

Entrez Gene IDs

3299 (Human); 26386 (Mouse)

Gene Symbol

HSF4

UniProt

BAA13433

Additional HSF4 Products

Product Documents for HSF4 Antibody (504524)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for HSF4 Antibody (504524)

For research use only

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