IGF-I R/IGF1R Antibody

R&D Systems | Catalog # AF-305-NA

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Rabbit, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Cell Culture, Functional Assay, Luciferase Reporter Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IGF-I R/IGF1R Primary Antibodies »

Product Specifications

Immunogen

Recombinant Human IGF-I R/IGF1R extracellular domain
Accession # P08069

Specificity

Detects human and mouse IGF-I R/IGF1R in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25-50% cross-reactivity with recombinant mouse IGF-I R is observed. In direct ELISAs, less than 1% cross-reactivity with recombinant human IGF‑II R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for IGF-I R/IGF1R Antibody

IGF-I R/IGF1R antibody in MCF-7 and HDLM-2 Human Cell Lines by Immunocytochemistry (ICC).

IGF-I R/IGF1R in MCF‑7 and HDLM-2 Human Cell Lines.

IGF-I R/IGF1R was detected in immersion fixed MCF-7 human breast cancer cell line (positive control, left panel) and HDLM-2 human Hodgkin's lymphoma cell line (negative control, right panel) using Goat Anti-Human/Mouse IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocols for Fluorescent ICC Staining of Cells on Coverslipsand Fluorescent ICC Staining of Non-adherent Cells.
IGF-I R/IGF1R antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

IGF-I R/IGF1R in Mouse Embryo.

IGF-I R/IGF1R was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
IGF-I R/IGF1R antibody in Human Placenta by Immunohistochemistry (IHC-P).

IGF-I R/IGF1R in Human Placenta.

IGF-I R/IGF1R was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 15 µg/mL Goat Anti-Human IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF-I R/IGF1R Antibody.

Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF-I R/IGF1R Antibody.

Recombinant Human IGF-I (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA). The ND50 is typically 0.5-1.5 µg/mL.
Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Representative H&E stained sections of hyperplasia (A) and normal mammary ducts (B) that developed following transplantation of pubertal MTB-IGFIR mammary epithelial cells that did not progress to palpable tumors.Immunostaining for IGF-IR (C, D) revealed high levels of expression with normal mammary ducts indicating successful engraftment of transgenic tissue. Scale bars, 200 µM (A–C) and 100 µM (D). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Representative H&E stained sections of hyperplasia (A) and normal mammary ducts (B) that developed following transplantation of pubertal MTB-IGFIR mammary epithelial cells that did not progress to palpable tumors.Immunostaining for IGF-IR (C, D) revealed high levels of expression with normal mammary ducts indicating successful engraftment of transgenic tissue. Scale bars, 200 µM (A–C) and 100 µM (D). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Immunohistochemistry for the IGF-IR (brown stain) in mammary epithelial cells of a 2 day old female mice captured at 100x (A) or 200x (B) magnification.Scale bars, 200 µM (A) and 100 µM (B). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Immunohistochemistry for the IGF-IR (brown stain) in mammary epithelial cells of a 2 day old female mice captured at 100x (A) or 200x (B) magnification.Scale bars, 200 µM (A) and 100 µM (B). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Detection of Mouse IGF-I R/IGF1R by Immunohistochemistry

Immunohistochemistry for the IGF-IR (brown stain) in mammary epithelial cells of a 2 day old female mice captured at 100x (A) or 200x (B) magnification.Scale bars, 200 µM (A) and 100 µM (B). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human IGF-I R ELISA Standard Curve

Human IGF-I R ELISA Standard Curve

Recombinant Human IGF-I R/IGF1R (Catalog # 391-GR) was serially diluted and captured by Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for IGF-I R/IGF1R Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) and immersion fixed frozen sections of mouse embryo

Western Blot

0.1 µg/mL
Sample: Recombinant Human IGF‑I R/IGF1R (Catalog # 391-GR) and Recombinant Mouse IGF‑I R/IGF1R (Catalog # 6630-GR).

Neutralization

Measured by its ability to neutralize IGF-I-induced proliferation in the MCF-7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 6 ng/mL Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1).

Reviewed Applications

Read 3 reviews rated 4 using AF-305-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References

  1. Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

UniProt

P08069

Additional IGF-I R/IGF1R Products

Product Documents for IGF-I R/IGF1R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for IGF-I R/IGF1R Antibody

For research use only

Citations for IGF-I R/IGF1R Antibody

Customer Reviews for IGF-I R/IGF1R Antibody (3)

4 out of 5
3 Customer Ratings
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4 Stars
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Customer Images

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  • Human/Mouse IGF-I R/IGF1R Antibody
    Name: Anonymous
    Application: Western Blot
    Verified Customer | Posted 12/18/2023
    IGF-I R/IGF1R Antibody AF-305-NA
  • Human/Mouse IGF-I R/IGF1R Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Cancer Tissue
    Species: Human
    Verified Customer | Posted 06/18/2021
    IGF-I R/IGF1R Antibody AF-305-NA
  • Human/Mouse IGF-I R/IGF1R Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Intestinal villi
    Species: Mouse
    Verified Customer | Posted 07/30/2019
    IGF1R in green
    IGF-I R/IGF1R Antibody AF-305-NA

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