Nurr1/NGFI-B beta/NR4A2 Antibody

R&D Systems | Catalog # AF2156

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Nurr1/NGFI-B beta/NR4A2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Nurr1/NGFI‑B beta /NR4A2
Val332-Lys558
Accession # Q06219

Specificity

Detects human and mouse Nurr1/NGFI‑B beta /NR4A2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Nurr1/NGFI-B beta/NR4A2 Antibody

Nurr1/NGFI‑B beta /NR4A2 in HeLa Human Cell Line.

Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Nurr1/NGFI‑B beta /NR4A2 in Mouse Brain (Hippocampus).

Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of fincorouzovyalomrgkg.jpg by Immunocytochemistry/ Immunofluorescence

Detection of fincorouzovyalomrgkg.jpg by Immunocytochemistry/ Immunofluorescence

RNA-seq from the peri-infarct cortex of ISP treated versus Veh-treated strokemice shows differentially regulated genes(a) DEGs that can be clustered into GO pathways (B) such as regulatorsof apoptotic signaling pathways, axon development pathways, and pathways thatare involved in responses to stress.(C) Validation of the top selected gene expression by qRT-PCR (n = 7 foreach group).(D–G) Nurr1 (Nr4a2) expression is decreased in peri-infarctcortex in stroke mice (arrows in D) but partially restored in ISP-treated mice(n = 4 for each group). Nurr1 expression is mainly detected in theNeuN+ neurons in the peri-infarct zone.(H–J) Bcl2 expression is upregulated in ISP-treated peri-infarctzone enriched in Iba1+ cells not GFAP+ reactive astrocytes(n = 4 for each group). *p < 0.05, **p < 0.01, ***p <0.001, Student’s t test for (C) and 2-way ANOVA for (J) and (G). Scalebar=50 μm.See Table S2for complete list of DEGs. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2211124722009469), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of jjgjbaa1wgvxctil5wru.jpg by Immunohistochemistry

Detection of jjgjbaa1wgvxctil5wru.jpg by Immunohistochemistry

Rsp neurons lose their identity in Satb2 CKO mice. FN1 is restricted to the SubC of controls (a) but expressed by neurons in the Rsp region of Satb2 CKO mice at P4 (b). c–h ER81, Citp2, and Nr4a2 are expressed in the SubC and specific layers of the Rsp in control mice (c, e, g). In Satb2 CKO mice, however, they are expressed throughout the Rsp regions while remain largely unchanged in the SubC (d, f, h). Cbln1 shows restricted expression in the Rsp of control mice (i), but its expression is lost in Satb2 CKO mice (j). Zbtb20 is expressed in superficial Rsp and CA1 of control mice (k), but undetectable in the Rsp region while remains unchanged in the CA1 of Satb2 CKO mice (l). Note that ectopic Zbtb20+ cells (arrows) are present in the subicular region of Satb2 CKO mice. Mef2C is intensely expressed in the Rsp and weakly in the SubC of control mice (m), but its expression was largely reduced in the Rsp region to a comparable cellular density of the SubC in Satb2 CKO mice (n). Dkk3 is strongly expressed in upper Rsp of control mice (o) but absent in the Rsp region of Satb2 CKO mice (p). Intense Lmo4 expression is reduced in the Rsp regions with a similar intensity to that in the SubC in Satb2 CKO mice (r) compared with controls (q). s, t Diagram of Rsp fate change in Satb2 CKO mice. DG dentate gyrus. n = 3 mice for each genotype. Scale bar = 500 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31666685), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Nurr1/NGFI-B beta/NR4A2 by Immunohistochemistry

Detection of Nurr1/NGFI-B beta/NR4A2 by Immunohistochemistry

RNA-seq from the peri-infarct cortex of ISP treated versus Veh-treated strokemice shows differentially regulated genes(a) DEGs that can be clustered into GO pathways (B) such as regulatorsof apoptotic signaling pathways, axon development pathways, and pathways thatare involved in responses to stress.(C) Validation of the top selected gene expression by qRT-PCR (n = 7 foreach group).(D–G) Nurr1 (Nr4a2) expression is decreased in peri-infarctcortex in stroke mice (arrows in D) but partially restored in ISP-treated mice(n = 4 for each group). Nurr1 expression is mainly detected in theNeuN+ neurons in the peri-infarct zone.(H–J) Bcl2 expression is upregulated in ISP-treated peri-infarctzone enriched in Iba1+ cells not GFAP+ reactive astrocytes(n = 4 for each group). *p < 0.05, **p < 0.01, ***p <0.001, Student’s t test for (C) and 2-way ANOVA for (J) and (G). Scalebar=50 μm.See Table S2for complete list of DEGs. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2211124722009469), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Nurr1/NGFI-B beta/NR4A2 by Immunohistochemistry

Detection of Nurr1/NGFI-B beta/NR4A2 by Immunohistochemistry

Satb2 determines Rsp fate by repressing Ctip2 and Nr4a2. a Diagram of Satb2-binding MARs in Nr4a2 genomic locus. In vivo ChIP assay using embryonic mouse cortex shows a remarkable enrichment of Satb2-binding MARs in Nr4a2 locus. *p < 0.05, **p < 0.01 (n = 3), error bars represent S.E.M. b Luciferase reporter assay shows that the transcriptional activities of Ctip2-A4 (positive control) and four Nr4a2 MARs are significantly reduced in the presence of Satb2. **p < 0.01 (n = 3), error bars represent S.E.M. c–i′ Ctip2- and/or Nr4a2-expression plasmids together with GFP are delivered into Rsp neurons by in utero electroporation in wild-type mice at E14.5 and pups are analyzed at P7. To make signals more clearly to compare, mRNA signals were photographed first, and then immunostaining of GFP was performed and photographed again. c, c′ Ctip2 is misexpressed in wild-type Rsp, and some of them are clumped. Inserts show Ctip2 expression in GFP+ cells (arrowheads). d, d′ A few Ctip2-misexpressed neurons contain FN1 transcripts and they often reside in ventral Rsp (arrowheads). Most of Rsp neurons with misexpression of Nr4a2 are arrested in VZ (e), and none of them contains FN1 transcripts in wild-type Rsp (f). Rsp neurons with co-misexpression of Ctip2 and Nr4a2 initiate FN1 expression in the VZ of Rsp (g, g′) and neocortical region (h, h′). i, i′ EphA6 is also ectopically induced in wild-type Rsp neurons when Ctip2 and Nr4a2 are co-misexpressed. VZ ventricular zone; Ncx neocortex. n = 3 mice for each group. Scale bars = 200 μm in c′, d′, and i′, 50 μm in inserts of c′, d′, and i′, 400 μm in f, and 100 μm in inserts of f Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31666685), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Nurr1/NGFI-B beta/NR4A2 by Immunocytochemistry/ Immunofluorescence

Detection of Nurr1/NGFI-B beta/NR4A2 by Immunocytochemistry/ Immunofluorescence

RNA-seq from the peri-infarct cortex of ISP treated versus Veh-treated strokemice shows differentially regulated genes(a) DEGs that can be clustered into GO pathways (B) such as regulatorsof apoptotic signaling pathways, axon development pathways, and pathways thatare involved in responses to stress.(C) Validation of the top selected gene expression by qRT-PCR (n = 7 foreach group).(D–G) Nurr1 (Nr4a2) expression is decreased in peri-infarctcortex in stroke mice (arrows in D) but partially restored in ISP-treated mice(n = 4 for each group). Nurr1 expression is mainly detected in theNeuN+ neurons in the peri-infarct zone.(H–J) Bcl2 expression is upregulated in ISP-treated peri-infarctzone enriched in Iba1+ cells not GFAP+ reactive astrocytes(n = 4 for each group). *p < 0.05, **p < 0.01, ***p <0.001, Student’s t test for (C) and 2-way ANOVA for (J) and (G). Scalebar=50 μm.See Table S2for complete list of DEGs. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2211124722009469), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Nurr1/NGFI-B beta/NR4A2 by Immunohistochemistry

Detection of Nurr1/NGFI-B beta/NR4A2 by Immunohistochemistry

Rsp neurons lose their identity in Satb2 CKO mice. FN1 is restricted to the SubC of controls (a) but expressed by neurons in the Rsp region of Satb2 CKO mice at P4 (b). c–h ER81, Citp2, and Nr4a2 are expressed in the SubC and specific layers of the Rsp in control mice (c, e, g). In Satb2 CKO mice, however, they are expressed throughout the Rsp regions while remain largely unchanged in the SubC (d, f, h). Cbln1 shows restricted expression in the Rsp of control mice (i), but its expression is lost in Satb2 CKO mice (j). Zbtb20 is expressed in superficial Rsp and CA1 of control mice (k), but undetectable in the Rsp region while remains unchanged in the CA1 of Satb2 CKO mice (l). Note that ectopic Zbtb20+ cells (arrows) are present in the subicular region of Satb2 CKO mice. Mef2C is intensely expressed in the Rsp and weakly in the SubC of control mice (m), but its expression was largely reduced in the Rsp region to a comparable cellular density of the SubC in Satb2 CKO mice (n). Dkk3 is strongly expressed in upper Rsp of control mice (o) but absent in the Rsp region of Satb2 CKO mice (p). Intense Lmo4 expression is reduced in the Rsp regions with a similar intensity to that in the SubC in Satb2 CKO mice (r) compared with controls (q). s, t Diagram of Rsp fate change in Satb2 CKO mice. DG dentate gyrus. n = 3 mice for each genotype. Scale bar = 500 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31666685), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Nurr1/NGFI-B beta/NR4A2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse brain (hippocampus)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Nurr1/NGFI‑B beta /NR4A2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nurr1/NGFI-B beta/NR4A2

Nurr1 belongs to the nerve growth factor-induced clone B subfamily of nuclear receptor superfamily and is designated NR4A2 in the Unified Nomenclature System for NRs. Nurr1 can function as a ligand-independent transcription factor. Nurr1 also heterodimerizes with retinoid X receptors (RXRs) to function as ligand-regulated transcription factor. Nurr1 is important for specification of dopaminergic neurons that degenerate in Parkinson’s disease. Human and mouse Nurr1 share 99% amino acid sequence homology.

Long Name

Nur-related Factor 1/Nerve Growth Factor Induced B beta/Nuclear Receptor Subfamily 4, Group A, 2

Alternate Names

NGFI-B beta, NOT, NR4A2, TINUR

Entrez Gene IDs

4929 (Human); 18227 (Mouse)

Gene Symbol

NR4A2

UniProt

Q06219

Additional Nurr1/NGFI-B beta/NR4A2 Products

Product Documents for Nurr1/NGFI-B beta/NR4A2 Antibody

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Product Specific Notices for Nurr1/NGFI-B beta/NR4A2 Antibody

For research use only

Citations for Nurr1/NGFI-B beta/NR4A2 Antibody

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