phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antibody (1019B)

R&D Systems | Catalog # MAB892

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1019B
View all RSK1/RSK2 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human RSK1 S221 site

Specificity

Detects human and mouse RSK1 when phosphorylated at S221, and human and mouse RSK2 when phosphorylated at S227.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antibody (1019B)

Detection of Human Phospho-RSK1 (S221)/RSK2 (S227) antibody by Western Blot.

Detection of Human Phospho-RSK1 (S221)/RSK2 (S227) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Monoclonal Antibody (Catalog # MAB892) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phopho-RSK1 (S221)/RSK2 (S227) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-RSK1 (S221)/RSK2 (S227) antibody by Simple WesternTM.

Detection of Human Phospho-RSK1 (S221)/RSK2 (S227) by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, loaded at 0.1 mg/mL. A specific band was detected for Phospho-RSK1 (S221)/RSK2 (S227) at approximately 91 kDa (as indicated) using 5 μg/mL of Rabbit Anti-Human/Mouse Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Monoclonal Antibody (Catalog # MAB892). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antibody (1019B)

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA

Reviewed Applications

Read 1 review rated 5 using MAB892 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RSK1/RSK2

RSK1 (Ribosomal S6 Kinase 1), gene name RPS6KA1 (Ribosomal Protein S6 Kinase alpha 1), also known as p90RSK1 (90 kDa RSK1) or MAPKAPK 1a (MAPK Activated Protein Kinase 1a), is a widely expressed member of the RSK family of growth factor-regulated serine/threonine kinases. RSK proteins contain two non-identical kinase catalytic domains, and mediate activation of Mitogen-Activated Kinase (MAPK) cascades and stimulation of cell proliferation and differentiation. Interaction with Erk1 and Erk2 initiates an ordered phosphorylation sequence which regulates RSK activation.  Phosphorylation of Ser 380 recruites PDK1 which, in turn, phosphorylates RSK1 in the activation loop at Ser 221 (or Ser227 in RSK2).

Long Name

Ribosomal Protein S6 Kinase II

Additional RSK1/RSK2 Products

Product Documents for phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antibody (1019B)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antibody (1019B)

For research use only

Customer Reviews for phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antibody (1019B) (1)

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  • Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody
    Name: Khuynh Nguyen
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 04/18/2022
    phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antibody (1019B) MAB892

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