Proprotein Convertase 2/PCSK2 Antibody

R&D Systems | Catalog # AF6018

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Proprotein Convertase 2/PCSK2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Proprotein Convertase 2/PCSK2
Gly110-Asn638
Accession # P16519

Specificity

Detects human Proprotein Convertase 2/PCSK2 in direct ELISAs and, human and mouse Proprotein Convertase 2/PCSK2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Proprotein Convertase 2/PCSK2 Antibody

Detection of Human and Mouse Proprotein Convertase 2/PCSK2 antibody by Western Blot.

Detection of Human and Mouse Proprotein Convertase 2/PCSK2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, beta TC-6 mouse beta cell insulinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Proprotein Convertase 2/PCSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6018) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Proprotein Convertase 2/PCSK2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Proprotein Convertase 2/PCSK2 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Proprotein Convertase 2/PCSK2 in HepG2 Human Cell Line.

Proprotein Convertase 2/PCSK2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human/Mouse Proprotein Convertase 2/PCSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue).This application has not been tested in mouse samples. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Proprotein Convertase 2/PCSK2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Proprotein Convertase 2/
PCSK2 (Catalog # 6018-SE), see our available Western blot detection antibodies.

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line, beta TC‑6 mouse beta cell insulinoma cell line, and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Proprotein Convertase 2/PCSK2

Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic amino acid residues (1). Autoactivation of PCSK2 occurs in a post‑Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. Within the region used as an immunogen, human PCSK2 shares 97% aa identity with mouse PCSK2.

References

  1. Zhou, A. et al. (1999) J. Biol. Chem. 274:20745.
  2. Lamango, N.S. et al. (1999) Arch. Biochem. Biophys. 362:275.
  3. Zhu, X. and I. Lindberg (1995) J. Cell Biol. 129:1641.
  4. Lamango, N. et al. (1996) Arch. Biochem. Biophys. 330:238.
  5. Rouillé, Y. et al. (1995) Front. Neuroendocrinol. 16:322.

Long Name

Proprotein Convertase Subtilisin/Kexin Type 2

Alternate Names

NEC2, PC2, PCSK2, SPC2

Entrez Gene IDs

5126 (Human); 18549 (Mouse); 25121 (Rat)

Gene Symbol

PCSK2

UniProt

P16519 (Human)

Additional Proprotein Convertase 2/PCSK2 Products

Product Documents for Proprotein Convertase 2/PCSK2 Antibody

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Product Specific Notices for Proprotein Convertase 2/PCSK2 Antibody

For research use only

Citations for Proprotein Convertase 2/PCSK2 Antibody

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Protocols

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