14-3-3 beta Antibody

R&D Systems | Catalog # AF4724

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all 14-3-3 beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human 14-3-3 beta
Met1-Asn246
Accession # P31946

Specificity

Detects human, mouse, and rat 14-3-3 beta in Western blots. Does not cross-react with other recombinant human 14-3-3 isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 14-3-3 beta Antibody

Detection of Human/Mouse/ Rat 14-3-3 beta antibody by Western Blot.

Detection of Human/Mouse/ Rat 14‑3‑3 beta by Western Blot.

Western blot shows lysates of TF-1 human erythroleukemic cell line cells, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat 14-3-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recom-binant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (20 ng/lane) were included. A specific band for 14-3-3 beta was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for 14-3-3 beta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: TF-1 human erythroleukemic cell line cells, mouse brain tissue, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 14-3-3 beta

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes - alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta / delta.

Alternate Names

1433 beta, GW128, HS1, YWHAB

Entrez Gene IDs

7529 (Human); 54401 (Mouse); 56011 (Rat)

Gene Symbol

YWHAB

UniProt

P31946

Additional 14-3-3 beta Products

Product Documents for 14-3-3 beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for 14-3-3 beta Antibody

For research use only

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