AMPK alpha 1 Antibody (362532)

R&D Systems | Catalog # MAB3197

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 362532
View all AMPK alpha 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AMPK alpha 1
Lys349-Gln559
Accession # Q13131

Specificity

Detects human, mouse, and rat AMPK alpha 1. No cross‑reactivity with recombinant human AMPK alpha 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for AMPK alpha 1 Antibody (362532)

Detection of Human, Mouse, and Rat AMPKa1 antibody by Western Blot.

Detection of Human, Mouse, and Rat AMPK alpha 1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, DA3 mouse myeloma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat AMPKa1 Monoclonal Antibody (Catalog # MAB3197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for AMPKa1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
AMPKa1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

AMPK alpha 1 in MCF‑7 Human Cell Line.

AMPKa1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat AMPKa1 Monoclonal Antibody (Catalog # MAB3197) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human AMPKa1 antibody by Simple WesternTM.

Detection of Human AMPK alpha 1 by Simple WesternTM.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for AMPK alpha 1 at approximately 57 & 64 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat AMPK alpha 1 Monoclonal Antibody (Catalog # MAB3197). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for AMPK alpha 1 Antibody (362532)

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Simple Western

20 µg/mL
Sample: MCF‑7 human breast cancer cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, DA3 mouse myeloma cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AMPK alpha 1

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The catalytic alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinases LKB1 and CaMKK beta. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Entrez Gene IDs

5562 (Human)

Gene Symbol

PRKAA1

UniProt

Q13131

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 Antibody (362532)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for AMPK alpha 1 Antibody (362532)

For research use only

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

Citations for AMPK alpha 1 Antibody (362532)

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Protocols

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