APE Antibody (200913)

R&D Systems | Catalog # MAB1044

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 200913
View all APE Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human APE
Pro2-Leu318
Accession # P27695

Specificity

Detects human, mouse and rat APE in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for APE Antibody (200913)

Detection of Human/Mouse/Rat APE antibody by Western Blot.

Detection of Human/Mouse/Rat APE by Western Blot.

Western blot shows lysates of NRK rat normal kidney cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat APE Monoclonal Antibody (Catalog # MAB1044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APE at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for APE Antibody (200913)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: NRK rat normal kidney cell, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APE

Human APE (also known as Ref-1) is the apurinic/apyrimidinic (AP) endonuclease required for efficient DNA base excision repair (BER). Following the removal of a damaged base by a DNA glycosylase, APE cleaves the AP site to allow resynthesis and ligation to complete repair. In addition, APE/Ref-1 acts as a factor that regulates the redox state of multiple transcription factors, including c-Jun, c-Fos, NF‑ kappa B, and p53.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Entrez Gene IDs

328 (Human); 305605 (Rat)

Gene Symbol

APEX1

UniProt

P27695

Additional APE Products

Product Documents for APE Antibody (200913)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for APE Antibody (200913)

For research use only

Related Research Areas

Base Excision Repair

Citations for APE Antibody (200913)

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