CrkL Antibody (515405)

R&D Systems | Catalog # MAB5127

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 515405
View all CrkL Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CrkL
Met1-Glu303
Accession # P46109

Specificity

Detects human, mouse and rat CrkL in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CrkL Antibody (515405)

Detection of Human CrkL antibody by Western Blot.

Detection of Human CrkL by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CrkL Monoclonal Antibody (Catalog # MAB5127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CrkL at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat CrkL antibody by Western Blot.

Detection of Mouse and Rat CrkL by Western Blot.

Western blot shows lysates of BaF3 mouse pro-B cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CrkL Monoclonal Antibody (Catalog # MAB5127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CrkL at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for CrkL Antibody (515405)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 3 reviews rated 3.7 using MAB5127 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CrkL

CrkL is a widely-expressed 39 kDa adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL initiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and the immune response. Furthermore, this adaptor protein plays an essential role in Chronic Myelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein

Alternate Names

crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Entrez Gene IDs

1399 (Human); 12929 (Mouse); 287942 (Rat)

Gene Symbol

CRKL

UniProt

P46109

Additional CrkL Products

Product Documents for CrkL Antibody (515405)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for CrkL Antibody (515405)

For research use only

Related Research Areas

Apoptosis Adaptor Proteins
Interferons

Customer Reviews for CrkL Antibody (515405) (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse/Rat CrkL Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/03/2020
    CrkL Antibody (515405) MAB5127
  • Human/Mouse/Rat CrkL Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018
  • Human/Mouse/Rat CrkL Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/07/2018

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