DGCR2 Antibody (1019908)

R&D Systems | Catalog # MAB10443

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1019908
View all DGCR2 Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived human DGCR2
Glu22-Ala349
Accession # P98153

Specificity

Detects human, mouse, and rat DGCR2 in Western blots. Detects human DGCR2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for DGCR2 Antibody (1019908)

Detection of Human, Mouse, and Rat DGCR2 antibody by Western Blot.

Detection of Human, Mouse, and Rat DGCR2 by Western Blot.

Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat DGCR2 Monoclonal Antibody (Catalog # MAB10443) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DGCR2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for DGCR2 Antibody (1019908)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Hep3B human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and H4‑II‑E‑C3 rat hepatoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DGCR2

DiGeorge Syndrome Critical Region 2 (DGCR2), also known as IDD, DGS-C, LAN, and SEZ-12, is an adhesion receptor protein located on the long arm of chromosome 22 (1). The DGCR2 gene encodes an integral membrane protein, consisting of an extracellular domain, a single transmembrane region and a cytoplasmic tail (1). The mature extracellular domain (ECD) of DGCR2 contains both a C-type lectin domain and a cysteine-rich region similar to that of the low density lipoprotein receptor (LDLR) (1, 3). The mature ECD of human DGCR2 shares 93% and 92% amino acid sequence identity with mouse and rat, respectively. DGCR2 is expressed during neurodevelopment in human brain tissues (3). Deletion of the 22q11.2 region results in an extremely variable disorder called 22q11.2 deletion syndrome, with a phenotype ranging from very mild symptoms to severe intellectual disability, facial dysmorphism, heart defects, and urogenital abnormalities (4). Recent studies suggest that DGCR2 regulates critical steps of early cortico-genesis possibly through a Reelin-dependent mechanism. Deletion of DGCR2 has a pathogenic impact on cortical formation by reducing protein expression level, and it plays a critical role in vulnerability to schizophrenia (5). Furthermore, expression of DGCR2 together with USP18 gene may serve as a progonostic marker for muscle invasive bladder cancer survival in patients (5).

References

  1. Augusseau, S. et al. (1986) Hum. Genet. 74:206.
  2. Mugikura, S. et al. (2016) Biochem Biophys Rep. 5:120.
  3. Kajiwara, K. et al. (1996) Biochem. Biophyis. Res. Commun. 222:144.
  4. Vaisvilas, M. et al. (2018) Balkan J Med Genet. 21:87.
  5. Molinard-Chenu A. and Dayer A. (2017) Biol. Psych. 83:692.

Long Name

DiGeorge Syndrome Critical Region Gene 2

Alternate Names

KIAA0163, SEZ-12

Entrez Gene IDs

9993 (Human); 13356 (Mouse); 360742 (Rat)

Gene Symbol

DGCR2

UniProt

P98153

Additional DGCR2 Products

Product Documents for DGCR2 Antibody (1019908)

Certificate of Analysis

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Product Specific Notices for DGCR2 Antibody (1019908)

For research use only

Citations for DGCR2 Antibody (1019908)

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