DGK-gamma (Diacylglycerol kinase gamma) is a 90 kDa, 791 amino acid (aa), type I member of the eukaryocytic diacylglycerol kinase family of enzymes. DGK-gamma possesses EF-hand, C1/Cys-rich zinc finger and catalytic domains, and is highly enriched in the retina and in cerebellar Purkinje cells. Expression elsewhere is limited to inactive isoforms missing kinase domain aa 451‑475, or zinc finger aa 333‑372. Upon calcium and PKC gamma -mediated activation, it translocates to the plasma membrane. DGK-gamma is also found in the nucleus, where it may promote cell cycle progression. Over aa 1‑126, human DGK-gamma shares 83% and 79% aa identity with mouse and rat DGK-gamma, respectively.
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 613531
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Product Specifications
Immunogen
E. coli-derived recombinant human DGK‑ gamma
Gly2-Ser126
Accession # P49619
Gly2-Ser126
Accession # P49619
Specificity
Detects human, mouse, and rat DGK‑ gamma in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for DGK-gamma Antibody (613531)
Detection of Human, Mouse, and Rat DGK‑ gamma by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, C2C12 mouse myoblast cell line, C6 rat glioma cell line, and SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat DGK- gamma Monoclonal Antibody (Catalog # MAB6389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK- gamma at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for DGK-gamma Antibody (613531)
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MDA‑MB‑468 human breast cancer cell line, C2C12 mouse myoblast cell line, C6 rat glioma cell line, and SH‑SY5Y human neuroblastoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MDA‑MB‑468 human breast cancer cell line, C2C12 mouse myoblast cell line, C6 rat glioma cell line, and SH‑SY5Y human neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DGK-gamma
Long Name
Diacylglycerol Kinase, gamma 90kDa
Alternate Names
DAGK3, DGKG, DGKgamma
Gene Symbol
DGKG
UniProt
Additional DGK-gamma Products
Product Documents for DGK-gamma Antibody (613531)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for DGK-gamma Antibody (613531)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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