EEA1 Antibody (871546)

R&D Systems | Catalog # MAB8047

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 871546
View all EEA1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EEA1 
Asn1249-Gln1356
Accession # Q15075

Specificity

Detects human EEA1 in direct ELISAs and human, mouse, and rat EEA1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for EEA1 Antibody (871546)

Detection of Human, Mouse, and Rat EEA1 antibody by Western Blot.

Detection of Human, Mouse, and Rat EEA1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, JAR human choriocarcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat EEA1 Monoclonal Antibody (Catalog # MAB8047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EEA1 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EEA1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

EEA1 in HeLa Human Cell Line.

EEA1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat EEA1 Monoclonal Antibody (Catalog # MAB8047) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to early endosomes in cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for EEA1 Antibody (871546)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, JAR human choriocarcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 3 using MAB8047 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EEA1

EEA1 (Early Endosome Autoantigen 1; also known as Endosome-associated protein p162 and Zn-finger FYVE domain-containing protein 2) is a 170-180 kDa protein that serves as an identifying marker for early endosomes. It is ubiquitously expressed, and found in both the cytosol and on cellular membranes. Its activity has been described as that of a tethering factor which links endosomes to endocytic vesicles, allowing for their fusion via a SNARE complex. Normally, EEA1 exists as a homodimer in the cytoplasm and appears to make transient contacts with endosome membrane phosphatidylinositol. When endosome fusion is not required, EEA1 serves as a substrate for p97, promoting EEA1 dissociation and endosome independence. When endosome fusion is required, EEA1 interacts with NSF, resulting in its removal from a large endosome-associated complex and subsequent endosomal vesicle fusion. Human EEA1 is synthesized as a 1411 amino acid (aa) protein that contains one C2H2-type Zn finger region (aa 41-64) and one FYVE Zn finger domain (aa 1352-1410). There is one isoform variant that contains a nine aa substitution for aa 925-1411. Over aa 1249-1356, human EEA1 shares 99% and 100% aa sequence identity with mouse and rat EEA1, respectively.

Long Name

Early Endosome Antigen 1

Alternate Names

MST105, MSTP105, ZFYVE2

Entrez Gene IDs

8411 (Human); 216238 (Mouse); 314764 (Rat)

Gene Symbol

EEA1

UniProt

Q15075 (Human)

Additional EEA1 Products

Product Documents for EEA1 Antibody (871546)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for EEA1 Antibody (871546)

For research use only

Citations for EEA1 Antibody (871546)

Customer Reviews for EEA1 Antibody (871546) (1)

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Customer Images

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  • Human/Mouse/Rat EEA1 Antibody
    Name: Teddy Chan
    Application: Western Blot
    Sample Tested: Human microglia cell lysate
    Species: Human
    Verified Customer | Posted 02/08/2019
    Very weak reactivity. DO NOT boil samples prior to loading.
    EEA1 Antibody (871546) MAB8047

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Protocols

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