Fyn Antibody (361228)

R&D Systems | Catalog # MAB3574

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 361228
View all Fyn Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Fyn
Met1-Gly83
Accession # P06241

Specificity

Detects endogenous human, mouse and rat Fyn in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fyn Antibody (361228)

Detection of Human/Mouse/Rat Fyn antibody by Western Blot.

Detection of Human/Mouse/Rat Fyn by Western Blot.

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, C2C12 mouse myoblast cell line, CTLL-2 mouse cytotoxic T cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Fyn Monoclonal Antibody (Catalog # MAB3574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fyn at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Fyn antibody in Human PBMCs by Immunocytochemistry (ICC).

Fyn in Human PBMCs.

Fyn was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human/Mouse/Rat Fyn Monoclonal Antibody (Catalog # MAB3574) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Fyn Antibody (361228)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Western Blot

0.5 µg/mL
Sample: MOLT-4 human acute lymphoblastic leukemia cell line, C2C12 mouse myoblast cell line, CTLL-2 mouse cytotoxic T cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fyn

Fyn is a ubiquitously expressed member of the Src family of intracellular tyrosine kinases. Fyn activity is implicated in T cell receptor signaling, adhesion-mediated signaling, and regulation of brain function. Mice lacking fyn display various deficits, including impaired spatial memory, hypomyelination, and uncoordinated hippocampal structure, suggesting that Fyn is involved in multiple neural signaling pathways.

Long Name

FYN oncogene related to SRC, FGR, YES

Alternate Names

SLK, SYN

Entrez Gene IDs

2534 (Human)

Gene Symbol

FYN

UniProt

P06241

Additional Fyn Products

Product Documents for Fyn Antibody (361228)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Fyn Antibody (361228)

For research use only

Citations for Fyn Antibody (361228)

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways
VEGF - VEGF R2 Signaling Pathway Thumbnail