Glutathione Peroxidase 1/GPX1 Antibody

R&D Systems | Catalog # AF3798

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Transgenic Mouse, Transgenic Rat

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot, Simple Western, IF/IHC, Simple Plex Automated Immunoassay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Glutathione Peroxidase 1/GPX1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glutathione Peroxidase 1
Gly48-Ala201
Accession # P07203

Specificity

Detects endogenous human, mouse, and rat GPX1. In Western blots, this antibody shows no cross-reactivity with recombinant human GPX2 or GPX3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glutathione Peroxidase 1/GPX1 Antibody

Glutathione Peroxidase 1/GPX1 antibody in Rat Liver by Immunohistochemistry (IHC-Fr).

Glutathione Peroxidase 1/GPX1 in Rat Liver.

Glutathione Peroxidase 1/GPX1 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human/Mouse/Rat Glutathione Peroxidase 1 antibody by Western Blot.

Detection of Human/Mouse/Rat Glutathione Peroxidase 1 by Western Blot.

Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human and Mouse Glutathione Peroxidase 1/GPX1 antibody by Simple WesternTM.

Detection of Human and Mouse Glutathione Peroxidase 1/GPX1 by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 30 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human Glutathione Peroxidase 1/GPX1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Glutathione Peroxidase 1/GPX1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Glutathione Peroxidase 1/GPX1 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 24 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Glutathione Peroxidase 1/GPX1 by Western Blot

Detection of Mouse Glutathione Peroxidase 1/GPX1 by Western Blot

Induction of oxidative stress defense during muscle mild mitochondrial stress. (A) Skeletal muscle mRNA expression in quadriceps muscle of oxidative stress defense markers of 24 wks old animals (n = 8–10). (B and C) Representative immunoblots of glutathione peroxidase 1 (GPX1) and GPX4 from of cropped gels using alpha Tubulin as loading control (2 samples shown out of 4–5 analyzed per group). (D) Quantification of GPX1 and GPX4 protein expression in quadriceps muscle from 24 wks old mice (4–5 per group). (E) Skeletal muscle catalase activity of 24 wks old animals (n = 5–10). (F) Skeletal muscle total GPX activity of 24 wks old animals (n = 7–10). (G) Representative immunoblots of methylglyoxal advanced glycation end-products (AGEs) in quadriceps muscle from 24 wks old mice using alpha Tubulin ( alpha TUB) as loading control (2 samples shown out of 4–5 analyzed per group). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; *p < 0.05; **p < 0.01; ***p < 0.001. (H) Graphical summary of Nrf2-dependent and Nrf2-independent adaptive cell-autonomous stress response pathways during respiratory uncoupling-induced muscle (mild) mitochondrial stress. This cartoon was created using Servier Medical Art (https://smart.servier.com). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-20901-4), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Glutathione Peroxidase 1/GPX1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat liver

Knockout Validated

Glutathione Peroxidase 1/GPX1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Glutathione Peroxidase 1/GPX1 knockout HEK293T cell line.

Simple Western

10 µg/mL
Sample: Human liver tissue and mouse liver tissue

Western Blot

1 µg/mL
Sample: Human, mouse and rat liver tissue

Reviewed Applications

Read 1 review rated 4 using AF3798 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glutathione Peroxidase 1/GPX1

Glutathione Peroxidase 1 (GPX1), also known as Cellular Glutathione Peroxidase, is a 201 amino acid, 23 kDa member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX1 is a 92 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX1 is an important antioxidant enzyme and protects red blood cells and other cells from oxidative damage. Deficiencies in red cell GPX1 have been associated with hemolytic anemia. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

CGPx, GPX1, GSHPX1

Entrez Gene IDs

2876 (Human); 14775 (Mouse); 24404 (Rat)

Gene Symbol

GPX1

UniProt

P07203

Additional Glutathione Peroxidase 1/GPX1 Products

Product Documents for Glutathione Peroxidase 1/GPX1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Glutathione Peroxidase 1/GPX1 Antibody

For research use only

Citations for Glutathione Peroxidase 1/GPX1 Antibody

Customer Reviews for Glutathione Peroxidase 1/GPX1 Antibody (1)

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  • GPX1 in mouse lungs
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 06/03/2025
    Increase of GPX1 after smoke exposure
    Antibodies diluted in milk-free antibody diluent, 1:20. Incubation was done for 60min.
    Glutathione Peroxidase 1/GPX1 Antibody AF3798

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