ILK Antibody (443208)

R&D Systems | Catalog # MAB4266

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 443208
View all ILK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ILK
Met1-Lys452
Accession # Q13418

Specificity

Detects human, mouse, and rat ILK in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for ILK Antibody (443208)

Detection of Human/Mouse/Rat ILK antibody by Western Blot.

Detection of Human/Mouse/Rat ILK by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat ILK Monoclonal Antibody (Catalog # MAB4266) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ILK (1 ng) was included. A specific band for ILK was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for ILK Antibody (443208)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PT18 mouse mast/basophil cell line

Reviewed Applications

Read 5 reviews rated 4.4 using MAB4266 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ILK

Integrin-linked kinase (ILK) is an ankyrin repeat-containing serine/threonine protein kinase that interacts with the cytoplasmic domain of beta 1 and beta 3 integrins. ILK functions with multiple binding partners to transmit signals from the extracellular matrix to actin filaments, regulating such processes as cellular attachment, cell cycle progression, and cytoskeletal organization. ILK kinase activity is induced by PI3 kinase and negatively regulated by the tumor suppressor PTEN.

Long Name

Integrin-linked Kinase

Alternate Names

ESTM24, p59

Entrez Gene IDs

3611 (Human); 16202 (Mouse); 170922 (Rat)

Gene Symbol

ILK

UniProt

Q13418

Additional ILK Products

Product Documents for ILK Antibody (443208)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for ILK Antibody (443208)

For research use only

Citations for ILK Antibody (443208)

Customer Reviews for ILK Antibody (443208) (5)

4.4 out of 5
5 Customer Ratings
5 Stars
60%
4 Stars
20%
3 Stars
20%
2 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Human/Mouse/Rat ILK Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    ILK Antibody (443208) MAB4266
  • Human/Mouse/Rat ILK Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/08/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    ILK Antibody (443208) MAB4266
  • Human/Mouse/Rat ILK Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human/Mouse/Rat ILK Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Trabecular meshwork
    Species: Human
    Verified Customer | Posted 06/21/2018
    Green: F-actin Red: ILK Blue: Nuclear
    ILK Antibody (443208) MAB4266
  • Human/Mouse/Rat ILK Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/25/2018
    ILK Antibody (443208) MAB4266

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