MEK2 Antibody (300317)

R&D Systems | Catalog # MAB2855

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 300317
View all MEK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MEK2
Met1-Val400
Accession # P36507

Specificity

Detects human, mouse, and rat MEK2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MEK1 or rhMEK5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for MEK2 Antibody (300317)

Detection of Human/Mouse/Rat MEK2 antibody by Western Blot.

Detection of Human/Mouse/Rat MEK2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat MEK2 Monoclonal Antibody (Catalog # MAB2855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (5 ng/lane) were included. A specific band for MEK2 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
MEK2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

MEK2 in HeLa Human Cell Line.

MEK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat MEK2 Monoclonal Antibody (Catalog # MAB2855) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for MEK2 Antibody (300317)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell

Reviewed Applications

Read 1 review rated 5 using MAB2855 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MEK2

Mitogen-activated protein kinase kinase 2 (MEK2 or MAP2K2), also known as MKK2,belongs to the STE family of kinases. Both MEK2 and the related MEK1 aredual‑specificity kinases, phosphorylating and activating the mitogen-activated proteinkinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK2 by Raf occurs through phosphorylation at S222 and S226.

Long Name

Mitogen-activated Protein Kinase Kinase 2

Alternate Names

MAP2K2, MKK2

Entrez Gene IDs

5605 (Human); 26396 (Mouse)

Gene Symbol

MAP2K2

UniProt

P36507

Additional MEK2 Products

Product Documents for MEK2 Antibody (300317)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for MEK2 Antibody (300317)

For research use only

Customer Reviews for MEK2 Antibody (300317) (1)

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  • Human/Mouse/Rat MEK2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HeLa human cervical epithelial carcinoma cell line
    Species: Human
    Verified Customer | Posted 01/22/2022
    MEK2 Antibody (300317) MAB2855

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Protocols

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