p130Cas Antibody

R&D Systems | Catalog # AF5730

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all p130Cas Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p130Cas
Met66-Val412
Accession # P56945

Specificity

Detects endogenous human, mouse and rat p130Cas in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for p130Cas Antibody

Detection of Human/Mouse/Rat p130Cas antibody by Western Blot.

Detection of Human/Mouse/Rat p130Cas by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat p130Cas Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5730) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for p130Cas at approximately 105 to 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for p130Cas Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p130Cas

p130Cas (protein 130 kDa Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93 kDa, it runs anomalously at 105‑130 kDa in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31 kDa fragment promotes anoikis and cell death. Human p130Cas is 870 amino acids (aa) in length. It contains one SH3 domain (aa 3‑65), a Pro‑rich region (aa 74‑87), at least 10 phosphotyrosine (aa 128‑410) and four phosphoserine (aa 134‑639) sites, an SH3 binding motif (aa 635‑643) and a HLH dimerization segment that interacts with CASL.

Long Name

Breast Cancer Anti-estrogen Resistance 1

Alternate Names

BCAR1, CASS1, CRKAS

Entrez Gene IDs

9564 (Human); 12927 (Mouse); 25414 (Rat)

Gene Symbol

BCAR1

UniProt

P56945

Additional p130Cas Products

Product Documents for p130Cas Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for p130Cas Antibody

For research use only

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