p62/SQSTM1 Antibody (864807) [Biotin]

R&D Systems | Catalog # BAM8028

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 864807
View all p62/SQSTM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p62/SQSTM1
Asp368-Leu440
Accession # Q13501

Specificity

p62/SQSTM1 antibodies are ideal for immunocytochemistry colocalization studies in autophagosomes. The unconjugated antibody detects human p62/SQSTM1 in direct ELISAs, and human, mouse and rat p62/SQSTM1 in Western Blots

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for p62/SQSTM1 Antibody (864807) [Biotin]

p62/SQSTM1 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC).

p62/SQSTM1 in RAW 264.7 Mouse Cell Line.

p62/SQSTM1 was detected in formaldehyde fixed RAW 264.7 mouse monocyte/macrophage cell line treated with LPS using Mouse Anti-Human/Mouse/Rat p62/SQSTM1 Biotinylated Monoclonal Antibody (Catalog # BAM8028) at 8 µg/mL overnight at 4 °C. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for p62/SQSTM1 Antibody (864807) [Biotin]

Application
Recommended Usage

Immunocytochemistry

8 µg/mL
Sample: Formaldehyde fixed RAW 264.7 mouse monocyte/macrophage cell line treated with LPS

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p62/SQSTM1

SQSTM1 (Sequestrome-1), also known as p62, is a widely expressed, stress-inducible, multifunctional 62 kDa intracellular protein. The 440 amino acid (aa) human SQSTM1 contains multiple adaptor domains that allow interaction with proteins in NGF/NFkB and other signaling pathways (notably TRAF6, atypical protein kinase C family and Src family), polyubiquitin, proteasome subunits and many others. It contains numerous regulatory phosphorylation sites and a dimerization site. SQSTM1 shuttles ubiquitinylated proteins to the proteasome and is important in autophagy and apoptosis. Its dysregulation is associated with Paget’s disease of bone, Parkinson’s and Alzheimer’s diseases, and cancers. Within aa 344-440, which includes the ubiquitin-binding domain, human SQSTM1 shares 100% aa sequence identity with mouse and rat SQSTM1.

Long Name

Sequestosome 1

Alternate Names

A170, EBIAP, ORCA, OSIL, PDB3, Sequestosome 1, SQSTM1, ZIP3

Entrez Gene IDs

8878 (Human); 18412 (Mouse); 113894 (Rat)

Gene Symbol

SQSTM1

UniProt

Q13501

Additional p62/SQSTM1 Products

Product Documents for p62/SQSTM1 Antibody (864807) [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for p62/SQSTM1 Antibody (864807) [Biotin]

For research use only

Related Research Areas

Autophagy
NFkB Pathway

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