Peroxiredoxin 2 Antibody

R&D Systems | Catalog # AF3489

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Peroxiredoxin 2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Peroxiredoxin 2
Met1-Asn198
Accession # P32119

Specificity

The antibody detects endogenous human, mouse and rat Peroxiredoxin 2 in Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant human (rh) Peroxiredoxin 1 and rhPeroxiredoxin 4 is observed, approximately 10% cross-reactivity with rhPeroxiredoxin 3 is observed, and less than 1% cross‑reactivity with rhPeroxiredoxin 5 and rhPeroxiredoxin 6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Peroxiredoxin 2 Antibody

Detection of Human/Mouse/Rat Peroxiredoxin 2 antibody by Western Blot.

Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Detection of Human Peroxiredoxin 2 antibody by Simple WesternTM.

Detection of Human Peroxiredoxin 2 by Simple WesternTM.

Simple Western shows lysates of Exosome Standard (PC‑3) (NBP2-49856) and Raji human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Peroxiredoxin 2 at approximately 26 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Applications for Peroxiredoxin 2 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Exosome Standard (PC-3) (Catalog # NBP2-49856) and Raji human Burkitt's lymphoma cell line

Western Blot

0.2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Peroxiredoxin 2

Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.

Alternate Names

NKEF-B, PRDX2, PRX2, TDPX1, TSA

Entrez Gene IDs

7001 (Human); 21672 (Mouse); 29338 (Rat)

Gene Symbol

PRDX2

UniProt

P32119

Additional Peroxiredoxin 2 Products

Product Documents for Peroxiredoxin 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Peroxiredoxin 2 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Oxidative Stress

Citations for Peroxiredoxin 2 Antibody

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