PP2C beta/PPM1B Antibody

R&D Systems | Catalog # AF4396

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PP2C beta/PPM1B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PP2C beta
Met1-Gly378
Accession # O75688

Specificity

Detects endogenous human, mouse and rat PP2C beta in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PP2C alpha or PP2C delta.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for PP2C beta/PPM1B Antibody

Detection of Human/Mouse/Rat PP2C beta /PPM1B antibody by Western Blot.

Detection of Human/Mouse/Rat PP2C beta /PPM1B by Western Blot.

Western blot shows lysates of CHP-100 human neuroblastoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP2C beta /PPM1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PP2C beta/PPM1B at approximately 53 and 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for PP2C beta/PPM1B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: CHP-100 human neuroblastoma cell line and A20 mouse B cell lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PP2C beta/PPM1B

Protein phosphatase 2C beta (PP2C beta ), also called protein phosphatase, magnesium-dependent type 1B (PPM1B), preferentially dephosphorylates serine and threonine residues on proteins. There are five splice variants with sizes of 53, 43, 42, 36, and 21 kDa. This enzyme is resistant to inhibitors of other protein phosphatases such as okadaic acid and calyculin. Association of PP2C beta with I kappa B kinase inhibits its phosphorylating activity and is regulated by TNFalpha stimulation. PP2C beta is found in many cell types and tissues, especially muscle. The mRNA for PP2C beta accumulates in mature spermatocytes and metaphase-arrested, unfertilized oocytes. Knockout of PP2C beta in mice is lethal prior to embryo implantation.

Long Name

Protein Phosphatase 2C beta

Alternate Names

Pp2c2, PP2CB, PPM1B

Entrez Gene IDs

5495 (Human); 19043 (Mouse); 24667 (Rat)

Gene Symbol

PPM1B

UniProt

O75688

Additional PP2C beta/PPM1B Products

Product Documents for PP2C beta/PPM1B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PP2C beta/PPM1B Antibody

For research use only

Citations for PP2C beta/PPM1B Antibody

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